以碱性磷酸酶为标记酶的超微量酶联免疫吸附测定法检测血清骨钙素
Determination of osteocalcin in serum by an ultramicro-ELISA with alkaline phosphatase as marker enzyme.
作者信息
Wenz I, Reissmann R, Börnig H
机构信息
Department of Medicine, Friedrich-Schiller University, Jean, FRG.
出版信息
Biomed Biochim Acta. 1991;50(2):145-9.
PMID:1877974
Abstract
A competitive ELISA for osteocalcin in 10 microliters samples has been developed with alkaline phosphatase as a marker enzyme. The assay for osteocalcin has an inter-assay and intra-assay variability of 13% and 12%, respectively; the limit of detection is 3.6 pg per assay. The assay was used to measure osteocalcin serum levels in patients with chronic hemodialysis and several bone diseases. The method for the purification of osteocalcin is also described.
摘要
已开发出一种用于检测10微升样本中骨钙素的竞争性酶联免疫吸附测定法（ELISA），以碱性磷酸酶作为标记酶。骨钙素测定的批间和批内变异率分别为13%和12%；检测限为每次测定3.6皮克。该测定法用于测量慢性血液透析患者和几种骨病患者的血清骨钙素水平。文中还描述了骨钙素的纯化方法。
Zotero 插件