使用芳基异硫氰酸酯对红细胞中的血影蛋白进行疏水标记。
Hydrophobic labelling of spectrin in erythrocytes using arylisothiocyanates.
作者信息
Sikorski A F, Kuczek M, Nyczka Z, Kubiak Z J
出版信息
Biomed Biochim Acta. 1987;46(1):75-82.
PMID:3579888
Abstract
Labelling of spectrin with the hydrophobic probes 2-naphthylisothiocyanate and 4-[125I]-iodophenylisothiocyanate dispersed in lipid vesicles was studied. Although spectrin is a peripheral protein of the erythrocyte membrane, it was found to be labelled as a result of incubation of washed red blood cells with hydrophobic arylisothiocyanates.
摘要
研究了用分散在脂质囊泡中的疏水探针2-萘基异硫氰酸酯和4-[¹²⁵I]-碘苯基异硫氰酸酯对血影蛋白进行标记的情况。尽管血影蛋白是红细胞膜的外周蛋白，但发现用疏水性芳基异硫氰酸酯孵育洗涤过的红细胞后，血影蛋白会被标记。
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