细菌视紫红质与脂质相互作用的圆二色性研究。

Circular dichroism study of bacteriorhodopsin-lipid interaction.

作者信息

Nishiya T, Tabushi I, Maeda A

出版信息

Biochem Biophys Res Commun. 1987 Apr 29;144(2):836-40. doi: 10.1016/s0006-291x(87)80040-2.

Abstract

Delipidated bacteriorhodopsin purified from purple membrane of H. halobium was reconstituted with the circular dichroism active phospholipid. The observed circular dichroism spectra in the 450-700 nm region characteristic of bacteriorhodopsin showed the temperature dependence characterized by a midpoint at ca. 45 degrees C and this spectral change showed the disaggregation of bacteriorhodopsin trimer to monomer. The circular dichroism spectra in the 250-400 nm region characteristic of the azo chromophore of phospholipid exhibited a remarkable temperature dependence synchronized with the disaggregation of bacteriorhodopsin, suggesting that a large proportion of the phospholipid is present as boundary lipid.

摘要

从嗜盐菌紫色膜中纯化得到的脱脂细菌视紫红质，用具有圆二色性活性的磷脂进行了重构。在450 - 700 nm区域观察到的细菌视紫红质特有的圆二色光谱显示出温度依赖性，其特征中点约为45℃，这种光谱变化表明细菌视紫红质三聚体解聚为单体。在250 - 400 nm区域磷脂偶氮发色团特有的圆二色光谱表现出与细菌视紫红质解聚同步的显著温度依赖性，这表明大部分磷脂以边界脂质的形式存在。