Sherman W V, Caplan S R
Biochim Biophys Acta. 1978 May 10;502(2):222-31. doi: 10.1016/0005-2728(78)90044-0.
Purple membrane fragments from Halobacterium halobium were reconstituted with the native lipids replaced by dipalmitoyl phosphatidylcholine and by egg lecithin. In parallel studies the temperature dependence of bacteriorhodopsin phototransient lifetime and absorption dichroism and of in situ lipid microviscosity were determined; the former two by, respectively, conventional and polarization flash photometry, and the latter by observation of emission depolarization of an embedded fluorescent dye, 1,6-diphenyl-1,3,5-hexatriene. Discontinuities in lipid microviscosity profiles in native and egg lecithin purple membrane were reflected in both the photochemical cycle frequency and bacteriorhodopsin chromophore rotational mobility. The influence exerted by membrane-lipid viscosity appears to be a secondary effect, and points to the bacteriorhodopsin chromophoric group being situated in the protein interior.
来自嗜盐菌的紫色膜片段用二棕榈酰磷脂酰胆碱和卵磷脂取代天然脂质后进行了重构。在平行研究中，测定了细菌视紫红质光瞬态寿命、吸收二色性以及原位脂质微粘度的温度依赖性；前两者分别通过传统和偏振闪光光度法测定，后者通过观察嵌入的荧光染料1,6 - 二苯基 - 1,3,5 - 己三烯的发射去极化来测定。天然和卵磷脂紫色膜中脂质微粘度曲线的不连续性反映在光化学循环频率和细菌视紫红质发色团旋转迁移率上。膜 - 脂质粘度所施加的影响似乎是一种次要效应，这表明细菌视紫红质发色团位于蛋白质内部。
