Ueda N, Yamamoto S, Fitzsimmons B J, Rokach J
Biochem Biophys Res Commun. 1987 Apr 29;144(2):996-1002. doi: 10.1016/s0006-291x(87)80062-1.
Arachidonate 5-lipoxygenase purified from porcine leukocytes produced several more polar compounds from 5,15-dihydroperoxy-eicosatetraenoic acid added as such or generated from 15-hydroperoxy acid. These polar products with absorption maxima at 301-302 nm and shoulders at 289 nm and 316-317 nm were identified as 5S,6R,15S-11-cis-lipoxin A and its 6-epimer, all-trans-lipoxin A isomers, and all-trans-lipoxin B isomers. Most of these lipoxins were presumably degradation products of a 5,6-epoxy intermediate formed by the catalysis of leukotriene A synthase, an integral part of 5-lipoxygenase. The rate of the enzymatic lipoxin synthesis from 15-hydroperoxy acid was about 6% of arachidonate 5-oxygenation.
从猪白细胞中纯化得到的花生四烯酸5-脂氧合酶，以原样添加的5,15-二氢过氧化二十碳四烯酸或由15-氢过氧酸生成的该酸为底物，生成了几种极性更强的化合物。这些极性产物在301 - 302 nm处有最大吸收峰，在289 nm和316 - 317 nm处有肩峰，被鉴定为5S,6R,15S-11-顺式脂氧素A及其6-差向异构体、全反式脂氧素A异构体和全反式脂氧素B异构体。这些脂氧素中的大多数可能是由白三烯A合酶催化形成的5,6-环氧中间体的降解产物，白三烯A合酶是5-脂氧合酶的一个组成部分。从15-氢过氧酸进行酶促脂氧素合成的速率约为花生四烯酸5-加氧反应速率的6%。
