Milner R J, Lai C, Lenoir D, Nave K, Bakhit C, Malfroy B
Biochem Soc Symp. 1986;52:107-17.
The brain of an adult rat expresses approximately 30,000 different brain-specific mRNAs. To investigate their encoded proteins, we have selected cDNA clones corresponding to mRNAs expressed exclusively in rat brain, determined their nucleotide sequences and generated antisera against synthetic peptides mimicking short regions of the deduced protein sequences. The clone plB236 encodes a protein that defines a widely distributed neuronal system and may be the precursor for a family of novel neuropeptides. A second clone, plB208, encodes rat brain proteolipid protein, the major protein component of central nervous system myelin. These studies have also identified an 82 nucleotide genetic element called an ID (identifier) sequence that may be involved in the regulation of transcription of brain-specific genes.
成年大鼠的大脑表达约30,000种不同的脑特异性mRNA。为了研究它们编码的蛋白质，我们选择了与仅在大鼠大脑中表达的mRNA相对应的cDNA克隆，确定了它们的核苷酸序列，并针对模拟推导蛋白质序列短区域的合成肽产生了抗血清。克隆plB236编码一种蛋白质，该蛋白质定义了一个广泛分布的神经元系统，可能是一类新型神经肽家族的前体。第二个克隆plB208编码大鼠脑蛋白脂质蛋白，它是中枢神经系统髓磷脂的主要蛋白质成分。这些研究还鉴定出一种称为ID（标识符）序列的82个核苷酸的遗传元件，它可能参与脑特异性基因转录的调控。
