Hu Pinghui, Liu Guanghui, Sun Huaping, Wei Wei
Pinghui Hu, Department of Ophthalmology, Nanjing University of Traditional Chinese Medicine, Li Xiang Eye Hospital of Soochow University, Suzhou 215000, Jiangsu, China.
Guanghui Liu, Department of Ophthalmology, People's Hospital Affiliated to Fujian University of Traditional Chinese Medicine, Fuzhou 350004, Fujian, China.
Pak J Med Sci. 2022 May-Jun;38(5):1327-1332. doi: 10.12669/pjms.38.5.5092.
To detect the expressions of hypoxia-inducible factor 1α (HIF-1α) and microRNA-210 (miR-210) in the aqueous humor of patients with central retinal vein occlusion (CRVO) combined with macular edema, and explore their correlations with CRVO combined with macular edema.
A total of 65 patients (65 eyes) with CRVO combined with macular edema who were treated in the Lixiang Eye Hospital of Soochow University from April 2018 to March 2020 were selected as subjects (CRVO combined with macular edema group). Additionally, 74 patients (74 eyes) with cataract were selected during the same period as cataract control group. The expressions of HIF-1α mRNA and miR-210 in the aqueous humor were detected by quantitative real-time PCR (qRT-PCR). The levels of monocyte chemoattractant protein-1 (MCP-1), interleukin-6 (IL-6) and vascular endothelial growth factor (VEGF) in the aqueous humor were measured using enzyme-linked immunosorbent assay (ELISA). The correlations of HIF-1α mRNA and miR-210 in the aqueous humor of patients with CRVO combined with macular edema with vasoactive molecule levels, the diagnostic value of HIF-1α mRNA and miR-210 levels in the aqueous humor in CRVO combined with macular edema, as well as the factors influencing the occurrence of CRVO combined with macular edema were analyzed.
The levels of HIF-1α mRNA, miR-210, MCP-1, VEGF and IL-6 in the aqueous humor of the CRVO combined with macular edema group were higher than those of the cataract control group ( < 0.05). In the CRVO combined with macular edema group, HIF-1α mRNA and miR-210 levels in the aqueous humor were positively correlated ( = 0.522, < 0.05), and they were positively correlated with MCP-1, VEGF and IL-6 levels ( < 0.05). The area under the curve (AUC) of HIF-1α mRNA and miR-210 in the aqueous humor in diagnosing CRVO combined with macular edema was 0.888 and 0.866, the specificity was 95.9% and 85.1%, and the sensitivity was 76.9% and 80.0%, respectively. The AUC of their combination was 0.937, with the specificity of 93.2% and the sensitivity of 86.2%. HIF-1α, miR-210 and VEGF were the independent risk factors affecting the occurrence of CRVO combined with macular edema ( < 0.05).
In patients with CRVO combined with macular edema, HIF-1α mRNA and miR-210 were highly expressed in the aqueous humor, which may play an important role in the occurrence and development of the disease.
检测视网膜中央静脉阻塞（CRVO）合并黄斑水肿患者房水中缺氧诱导因子1α（HIF-1α）和微小RNA-210（miR-210）的表达，并探讨它们与CRVO合并黄斑水肿的相关性。
选取2018年4月至2020年3月在苏州大学理想眼科医院就诊的65例（65眼）CRVO合并黄斑水肿患者作为研究对象（CRVO合并黄斑水肿组）。另外，选取同期74例（74眼）白内障患者作为白内障对照组。采用定量实时荧光定量聚合酶链反应（qRT-PCR）检测房水中HIF-1α mRNA和miR-210的表达。采用酶联免疫吸附测定（ELISA）法检测房水中单核细胞趋化蛋白-1（MCP-1）、白细胞介素-6（IL-6）和血管内皮生长因子（VEGF）的水平。分析CRVO合并黄斑水肿患者房水中HIF-1α mRNA和miR-210与血管活性分子水平的相关性、房水中HIF-1α mRNA和miR-210水平对CRVO合并黄斑水肿的诊断价值以及影响CRVO合并黄斑水肿发生的因素。
CRVO合并黄斑水肿组房水中HIF-1α mRNA、miR-210、MCP-1、VEGF和IL-6水平高于白内障对照组（P<0.05）。在CRVO合并黄斑水肿组中，房水中HIF-1α mRNA与miR-210水平呈正相关（r=0.522，P<0.05），且它们与MCP-1、VEGF和IL-6水平呈正相关（P<0.05）。房水中HIF-1α mRNA和miR-210诊断CRVO合并黄斑水肿的曲线下面积（AUC）分别为0.888和0.866，特异性分别为95.9%和85.1%，敏感性分别为76.9%和80.0%。两者联合的AUC为0.937，特异性为93.2%，敏感性为86.2%。HIF-1α、miR-210和VEGF是影响CRVO合并黄斑水肿发生的独立危险因素（P<0.05）。
CRVO合并黄斑水肿患者房水中HIF-1α mRNA和miR-210高表达，可能在疾病的发生发展中起重要作用。
