Laboratorio de Biología Molecular e Inmunología de arbovirus, Unidad de Biomedicina, Facultad de Estudios Superiores Iztacala, Universidad Nacional Autónoma de México, Tlalnepantla, Mexico.
OaxacaLab Laboratorio de análisis Clínicos, Oaxaca, Mexico.
Front Cell Infect Microbiol. 2022 Jun 21;12:890750. doi: 10.3389/fcimb.2022.890750. eCollection 2022.
Dengue and Zika viruses cocirculate annually in endemic areas of Mexico, causing outbreaks of different magnitude and severity every year, suggesting a continuous selection of variants with variable phenotypes of transmissibility and virulence. To evaluate if variants with different phenotypes cocirculate during outbreaks, we isolated dengue and Zika viruses from blood samples of febrile patients from Oaxaca City during the 2016 and 2019 epidemic years. We compared their replication kinetics in human cells, susceptibility to type I interferon antiviral response, and the accumulation of subgenomic RNA on infected cells. We observed correlations between type I interferon susceptibility and subgenomic RNA accumulation, with high hematocrit percentage and thrombocytopenia. Our results suggest that that cocirculate in Oaxaca, Mexico, have variable sensitivity to the antiviral activity of type I interferons, and this phenotypic trait correlates with the severity of the disease.
登革热和寨卡病毒在墨西哥的流行地区每年同时流行，导致每年爆发不同程度和严重程度的疫情，这表明病毒不断发生变异，具有不同的传播力和毒力表型。为了评估在疫情爆发期间是否有不同表型的变异病毒同时流行，我们从 2016 年和 2019 年瓦哈卡市发热患者的血液样本中分离出登革热和寨卡病毒。我们比较了它们在人细胞中的复制动力学、对 I 型干扰素抗病毒反应的敏感性以及感染细胞中亚基因组 RNA 的积累。我们观察到 I 型干扰素敏感性和亚基因组 RNA 积累之间存在相关性，与高红细胞压积百分比和血小板减少症相关。我们的结果表明，在墨西哥瓦哈卡流行的登革热和寨卡病毒对 I 型干扰素的抗病毒活性具有不同的敏感性，这种表型特征与疾病的严重程度相关。
