从……进行内体循环囊泡的免疫分离
Immunoisolation of Endosomal Recycling Vesicles from .
作者信息
Suzuki Sho W, Emr Scott D
机构信息
Weill Institute for Cell and Molecular Biology and Department of Molecular Biology and Genetics, Cornell University, Ithaca, USA.
出版信息
Bio Protoc. 2022 May 5;12(9):e4403. doi: 10.21769/BioProtoc.4403.
Abstract
Endosomal recycling is essential for the appropriate function of the endosome. During this process, endosomal coat complexes (, retromer, and Mvp1) are recruited to the endosome, and deform its membrane to form recycling vesicles. To further analyze this, we developed a protocol for the immunoisolation of recycling vesicles from budding yeast. This method is a powerful way to characterize endosomal recycling pathways.
摘要
内体循环对于内体的正常功能至关重要。在此过程中，内体包被复合物（如回收体和Mvp1）被招募到内体，并使其膜变形以形成循环囊泡。为了进一步分析这一点，我们开发了一种从出芽酵母中免疫分离循环囊泡的方法。该方法是表征内体循环途径的有力手段。
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本文引用的文献
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A PX-BAR protein Mvp1/SNX8 and a dynamin-like GTPase Vps1 drive endosomal recycling.PX-BAR 蛋白 Mvp1/SNX8 和动力蛋白样 GTPase Vps1 驱动内体再循环。
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2
The ESCRT pathway.外体分选复合体（ESCRT）途径。
Dev Cell. 2011 Jul 19;21(1):77-91. doi: 10.1016/j.devcel.2011.05.015.
3
Retromer and the sorting nexins Snx4/41/42 mediate distinct retrieval pathways from yeast endosomes.逆转录酶复合物和分选连接蛋白Snx4/41/42介导酵母内体的不同回收途径。
EMBO J. 2003 Feb 3;22(3):548-57. doi: 10.1093/emboj/cdg062.
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