Li Zhuo, Li Shuangxi, Li Tianrui, Gao Xinxin, Zhu Liandong
School of Resources & Environmental Science, Hubei International Scientific and Technological Cooperation Base of Sustainable Resource and Energy, Hubei Key Laboratory of Biomass-Resources Chemistry and Environmental Biotechnology, Wuhan University, Wuhan 430079, P.R. China.
iScience. 2022 Jun 18;25(7):104638. doi: 10.1016/j.isci.2022.104638. eCollection 2022 Jul 15.
Microalgae-based technology is an effective and environmentally friendly method for antibiotics-contaminated wastewater treatment. To assess the tolerance and removal ability of to ciprofloxacin (CIP), this study comprehensively revealed the responses of to CIP exposure and its degradation processes through physiological and transcriptomic analyses. Although the photosynthetic system was inhibited, the growth of was not negatively affected by CIP. Dissolved organic matter was analyzed and indicated that humic-like substances were released to alleviate the stress of CIP. In addition, the maximum removal of CIP was 83.3% under 20 mg L CIP exposure. HPLC-MS/MS and RNA-Seq analyses suggested that CIP could be bioaccumulated and biodegraded by through the reactions of hydroxylation, demethylation, ring cleavage, oxidation, dehydrogenation, and decarboxylation with the help of intracellular oxidoreductases, especially cytochrome P450. Collectively, this research shows that have a great potential for removing CIP from wastewater.
基于微藻的技术是一种处理抗生素污染废水的有效且环保的方法。为评估[微藻名称]对环丙沙星（CIP）的耐受性和去除能力，本研究通过生理和转录组分析全面揭示了[微藻名称]对CIP暴露的响应及其降解过程。尽管光合系统受到抑制，但CIP并未对[微藻名称]的生长产生负面影响。对溶解有机物的分析表明，类腐殖质被释放出来以缓解CIP的胁迫。此外，在20 mg/L CIP暴露下，[微藻名称]对CIP的最大去除率为83.3%。HPLC-MS/MS和RNA-Seq分析表明，在细胞内氧化还原酶尤其是细胞色素P450的帮助下，[微藻名称]可通过羟基化、去甲基化、环裂解、氧化、脱氢和脱羧反应对CIP进行生物累积和生物降解。总体而言，本研究表明[微藻名称]在从废水中去除CIP方面具有巨大潜力。
