Guangdong Provincial Key Laboratory of Marine Resources and Coastal Engineering, School of Marine Sciences, Sun Yat-Sen University, Zhuhai 519082, China.
Guangdong Provincial Key Laboratory of Marine Resources and Coastal Engineering, School of Marine Sciences, Sun Yat-Sen University, Zhuhai 519082, China; Guangdong Institute for Cereal Science Research, Guangzhou 510050, China.
J Hazard Mater. 2022 Mar 5;425:128036. doi: 10.1016/j.jhazmat.2021.128036. Epub 2021 Dec 8.
Microalgae-based technology is an environmental-friendly and cost-effective method for treating antibiotics-contaminated wastewater. This work investigated the removal of levofloxacin (LEV) by an oleaginous microalgae Chromochloris zofingiensis under photoautotrophic and heterotrophic conditions. The results showed that the significantly higher biomass production, accumulation of extracellular polymeric substance and LEV removal efficiency were achieved in heterotrophic C. zofingiensis compared with the photoautotrophic ones. The removal efficiencies under the heterotrophic condition were 97%, 88% and 76% at 1, 10, and 100 mg/L LEV, respectively. HPLC-MS/MS and RNA-Seq analyses suggested that LEV could be bioaccumulated and biodegraded by heterotrophic C. zofingiensis through the reactions of defluorination, hydroxylation, demethylation, ring cleavage, oxidation, dehydrogenation, denitrification, and decarboxylation. The chemical composition of the algal biomass obtained after LEV treatment indicated the potential of this alga for removing LEV from wastewaters and simultaneously producing biodiesel, astaxanthin, and other products. Collectively, this research shows that the heterotrophic C. zofingiensis can be identified as a promising candidate for removing LEV in wastewater remediation.
基于微藻的技术是一种环保且经济有效的方法，可用于处理含有抗生素的废水。本工作研究了在自养和异养条件下产油微藻东海小球藻（Chromochloris zofingiensis）对左氧氟沙星（LEV）的去除。结果表明，与自养相比，异养东海小球藻具有更高的生物量生产、胞外聚合物的积累和 LEV 的去除效率。在异养条件下，LEV 的去除效率分别为 1、10 和 100mg/L 时的 97%、88%和 76%。HPLC-MS/MS 和 RNA-Seq 分析表明，LEV 可通过脱氟、羟基化、去甲基化、环裂解、氧化、脱氢、脱硝和脱羧等反应被异养东海小球藻生物积累和生物降解。LEV 处理后获得的藻生物质的化学成分表明，该藻类具有从废水中去除 LEV 并同时生产生物柴油、虾青素和其他产品的潜力。总的来说，这项研究表明，异养东海小球藻可以被鉴定为废水修复中去除 LEV 的一种有前途的候选生物。
