Wang Hongwei, Bu Xiaojie, Chen Fulian, Wang Yan, Chen Yao
Department of Health Care, People's Hospital of Rizhao, Rizhao, Shandong, PR China.
Department of Endocrinology, Affiliated Yidu Central Hospital of Weifang Medical College, Weifang, Shandong, PR China.
Histol Histopathol. 2022 Nov;37(11):1127-1141. doi: 10.14670/HH-18-492. Epub 2022 Jul 8.
Osteoporosis is a common disease in postmenopausal women characterized by systemic bone mass loss, microstructure fragility and increased incidence of fractures. Resistant dextrin (RD) is a soluble fiber with beneficial metabolic effects. However, the beneficial effect of RD in osteoporosis remains to be determined.
In this study, we investigated the effect of dietary RD supplement on osteoporosis in ovariectomized (OVX) rats. Both the control (sham) and OVX group rats were gavaged with RD (10 g/kg/d) or equal amount of saline for 12 weeks, and histological and biomechanical analyses were conducted to evaluate bone microstructure and strength. Furthermore, we also evaluated the effects of RD on osteoclastogenesis in bone marrow macrophages (BMMs) by detecting the expression of osteoclast-related genes using qRT-PCR and Western blot analysis.
The results showed that in OVX rats the bone strength and microstructure characteristics were significantly improved with RD supplement for 12 weeks. Additionally, the mRNA and protein expression of osteoclast markers, such as CTSK, NF-κB and NFATC1, were significantly down-regulated in BMMs isolated from RD supplement group. RD also suppressed RANKL-induced osteoclastogenesis in BMMs.
These findings suggest that RD ameliorates osteoporosis in OVX rats by inhibiting osteoclast differentiation. RD suppresses RANKL-induced osteoclastogenesis possibly through modulating Akt and NF-κB signaling pathways. These data indicate that a dietary supplement of RD might serve as an intervention strategy for menopausal osteoporosis.
骨质疏松症是绝经后女性的常见疾病，其特征为全身性骨量丢失、微观结构脆弱及骨折发生率增加。抗性糊精（RD）是一种具有有益代谢作用的可溶性纤维。然而，RD对骨质疏松症的有益作用仍有待确定。
在本研究中，我们调查了膳食补充RD对去卵巢（OVX）大鼠骨质疏松症的影响。对照组（假手术组）和OVX组大鼠均灌胃给予RD（10 g/kg/d）或等量生理盐水，持续12周，然后进行组织学和生物力学分析以评估骨微观结构和强度。此外，我们还通过qRT-PCR和蛋白质印迹分析检测破骨细胞相关基因的表达，评估RD对骨髓巨噬细胞（BMMs）中破骨细胞生成的影响。
结果显示，在OVX大鼠中，补充RD 12周后骨强度和微观结构特征得到显著改善。此外，从RD补充组分离的BMMs中，破骨细胞标志物如CTSK、NF-κB和NFATC1的mRNA和蛋白质表达显著下调。RD还抑制了BMMs中RANKL诱导的破骨细胞生成。
这些发现表明，RD通过抑制破骨细胞分化改善OVX大鼠的骨质疏松症。RD可能通过调节Akt和NF-κB信号通路抑制RANKL诱导的破骨细胞生成。这些数据表明，膳食补充RD可能作为绝经后骨质疏松症的一种干预策略。
