Tavares M A, Paula-Barbosa M M, Verwer R W
Alcohol. 1987 Mar-Apr;4(2):109-16. doi: 10.1016/0741-8329(87)90007-3.
The cerebellar molecular layer of chronic alcohol treated rats showed degenerated parallel fiber boutons and vacated Purkinje cell spines after 6 months of alcohol feeding; degenerated Purkinje cell dendrites were concomitantly observed. The number of synapses between parallel fibers and Purkinje cell spines decreased after 6 months whereas their mean synaptic diameter increased throughout the experiment. Conversely, synapses between parallel fibers and dendrites of interneurons increased in 18-month alcohol-fed group. Quantifications were done using a discrete unfolding procedure. It was also observed that some parallel fiber terminals simultaneously established synapses with multiple Purkinje cell spines and with dendrites of interneurons. These results were obtained from groups of 6 rats alcohol-fed for 1, 3, 6, 12 and 18 months and compared with age-matched pair-fed controls. Previous reports of alcohol-induced degenerative changes in the adult rat central nervous system were, thus, fully corroborated. Furthermore, there was evidence of remodeling processes pointing to compensatory plastic mechanisms in the cerebellar circuitry albeit not sufficient to overcome its alcohol-induced deterioration.
