Laboratorio Integral de Investigación en Alimentos, Tecnológico Nacional de México/I.T Tepic, Tepic, Mexico.
Hospital General del ISSSTE, San Luis Potosí, Mexico.
Photodermatol Photoimmunol Photomed. 2023 Jan;39(1):51-59. doi: 10.1111/phpp.12815. Epub 2022 Jul 12.
Ultraviolet B (UVB) causes photoaging of the skin, the appearance of wrinkles, spots, and alteration of the skin barrier. The main cells in the most superficial layer of the skin are the keratinocytes; these cells play an important role in protecting this organ.
The present study aimed to investigate the antioxidant activity of the hydrolysates from kafirin to inhibit UVB-induced responses in human keratinocytes cells (HaCaT).
Kafirin hydrolysates were produced by enzymatic hydrolysis with alcalase. The activity of superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx), in the HaCaT cell line in the presence of UVB and the effects of the hydrolysates against the UVB-induced response were evaluated. Furthermore, the peptides that were generated by hydrolysis were identified in silico using the BIOPEP database.
Two protein sequences were identified (α-kafirin and the precursor protein of α-kafirin), in the kafirin extract. A degree of hydrolysis of 18.8% was obtained by hydrolyzing the kafirin extract with alcalase. The kafirin hydrolysates avoided the decrease in endogenous antioxidant enzymes such as SOD, CAT, and GPx reducing the oxidative stress generated by UVB. Using the BIOPEP-UWM database, we found 102 peptide sequences, and it has shown that the peptides have a large amount of hydrophobic amino acids such as proline, alanine, and glutamine, and amino acids with high antioxidant capacity.
These results suggest that the kafirin hydrolysates can be used as antioxidant agents to ameliorate UVB-induced skin keratinocytes cells' response in vitro, providing an alternative against UVB-induced photoaging.
紫外线 B（UVB）会导致皮肤光老化，出现皱纹、斑点和皮肤屏障改变。皮肤最浅层的主要细胞是角质形成细胞；这些细胞在保护该器官方面起着重要作用。
本研究旨在探讨来自玉米醇溶蛋白的水解物的抗氧化活性，以抑制人角质形成细胞（HaCaT）中 UVB 诱导的反应。
采用碱性蛋白酶对玉米醇溶蛋白进行酶解制备水解物。在存在 UVB 的情况下，评估了水解物对 HaCaT 细胞中超氧化物歧化酶（SOD）、过氧化氢酶（CAT）和谷胱甘肽过氧化物酶（GPx）活性的影响，以及水解物对 UVB 诱导反应的抑制作用。此外，使用 BIOPEP 数据库对水解产生的肽进行了计算机模拟鉴定。
从玉米醇溶蛋白提取物中鉴定出两种蛋白序列（α-玉米醇溶蛋白和α-玉米醇溶蛋白前体蛋白）。用碱性蛋白酶水解玉米醇溶蛋白提取物可获得 18.8%的水解度。玉米醇溶蛋白水解物避免了内源性抗氧化酶（如 SOD、CAT 和 GPx）的减少，从而减少了 UVB 产生的氧化应激。使用 BIOPEP-UWM 数据库，我们发现了 102 条肽序列，表明这些肽具有大量疏水性氨基酸，如脯氨酸、丙氨酸和谷氨酰胺，以及具有高抗氧化能力的氨基酸。
这些结果表明，玉米醇溶蛋白水解物可用作抗氧化剂，以改善体外 UVB 诱导的皮肤角质形成细胞反应，为对抗 UVB 诱导的光老化提供了一种替代方法。
