Fan Guanghan, Li Ying, Ye Qinghua, Niu Qinya, Zhao Xinyu, Chen Ling, Gu Qihui, Zhang Youxiong, Wei Xianhu, Wu Shi, Wu Qingping, Wu Yuwei
National Health Commission Science and Technology Innovation Platform for Nutrition and Safety of Microbial Food, Guangdong Provincial Key Laboratory of Microbial Safety and Health, State Key Laboratory of Applied Microbiology Southern China, Institute of Microbiology, Guangdong Academy of Sciences, Guangzhou, China.
Department of Food Science and Engineering, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, China.
J Tissue Eng. 2024 Dec 6;15:20417314241300388. doi: 10.1177/20417314241300388. eCollection 2024 Jan-Dec.
Fetal bovine serum (FBS) plays a crucial role in the composition of animal cell culture medium. However, conventional serum-based medium face numerous challenges. The use of animal-derived free hydrolysate (ADFH) has garnered significant attention in research and applications as a viable alternative to FBS-containing medium in animal cell culture. This article provides a comprehensive overview of the effects, mechanisms of action, and applications of ADFH in animal cell culture. ADFH serves as an effective substitute for FBS-containing medium, enhancing various cellular processes, including cell proliferation, viability, protein synthesis, production, survival, and stability. Several mechanisms of action for ADFH have been elucidated through scientific investigations, such as nutrient provision, activation of signaling pathways, regulation of protein synthesis and folding, protection against oxidative damage and apoptosis, as well as cell cycle regulation. Researches and applications of ADFH represent a promising approach to overcoming the limitations of FBS-containing medium and advancing the field of animal cell culture. This review provides a theoretical foundation for promoting the development of sustainable and alternative hydrolysates, as well as the continued progress of animal cell culture.
胎牛血清（FBS）在动物细胞培养基的组成中起着至关重要的作用。然而，传统的基于血清的培养基面临诸多挑战。动物源游离水解产物（ADFH）作为动物细胞培养中含FBS培养基的一种可行替代品，在研究和应用中已受到广泛关注。本文全面综述了ADFH在动物细胞培养中的作用效果、作用机制及应用。ADFH可有效替代含FBS的培养基，增强包括细胞增殖、活力、蛋白质合成、生产、存活及稳定性等多种细胞过程。通过科学研究已阐明ADFH的几种作用机制，如提供营养、激活信号通路、调节蛋白质合成与折叠、抵御氧化损伤和细胞凋亡以及细胞周期调控等。ADFH的研究与应用是克服含FBS培养基局限性及推动动物细胞培养领域发展的一种有前景的方法。本综述为促进可持续和替代性水解产物的发展以及动物细胞培养的持续进步提供了理论基础。
