Department of Transfusion Medicine, General Hospital of Central Theater Command of the PLA, Wuhan 430070, Hubei, China.
The First School of Clinical Medicine, Southern Medical University, Guangzhou 510515, Guangdong, China.
Can J Microbiol. 2022 Jul 1;68(7):501-506. doi: 10.1139/cjm-2021-0314. Epub 2022 Jul 8.
, the causative agent of plague, is one of the most dangerous pathogens in the world. Both the cyclic AMP receptor protein (CRP) and ferric uptake regulator (Fur) are global regulators that control the expression of a great deal of genes involved in a variety of cellular functions in . In this work, two CRP box-like deoxyribonucleic acid (DNA) sequences were detected in the upstream DNA region of , suggesting that the transcription of might be directly regulated by CRP in . Thus, transcriptional regulation of by CRP was investigated by primer extension, quantitative real-time PCR, LacZ fusion, and electrophoretic mobility shift assays. The results demonstrated that CRP was able to bind the regulatory DNA region of to activate its transcription. The data presented here not only suggested that the CRP and Fur regulons were bridged together via the direct regulation of by CRP, but also provided us a deeper understanding of the transcriptional regulation of in .
鼠疫耶尔森菌是世界上最危险的病原体之一。环磷酸腺苷受体蛋白（CRP）和铁摄取调节蛋白（Fur）都是全球调节因子，控制着大量与各种细胞功能相关的基因的表达。在这项工作中，在 的上游 DNA 区域检测到了两个 CRP 盒样脱氧核糖核酸（DNA）序列，这表明 在 中可能直接受 CRP 调控。因此，通过引物延伸、实时定量 PCR、LacZ 融合和电泳迁移率变动分析研究了 CRP 对 的转录调控。结果表明，CRP 能够结合 的调节 DNA 区域来激活其转录。这里呈现的数据不仅表明 CRP 和 Fur 调控子通过 CRP 对 的直接调控联系在一起，而且还使我们更深入地了解 在 中的转录调控。
