Faculty of Natural Sciences and Mathematics, University of Maribor, Koroška 160, 2000 Maribor, Slovenia.
Faculty of Education, University of Maribor, Koroška Cesta 160, 2000 Maribor, Slovenia.
Biomacromolecules. 2022 Aug 8;23(8):3452-3457. doi: 10.1021/acs.biomac.2c00593. Epub 2022 Jul 8.
The application of highly porous and 3D interconnected microcellular polyelectrolyte polyHIPE (PE-PH) monoliths based on (3-acrylamidopropyl)-trimethylammonium chloride as soilless cultivation substrates for in vitro embryo culture is discussed. The embryo axes isolated from chickpea seeds are inoculated onto the surface of the monoliths and allowed to germinate. Germination study show that the newly disclosed PE-PH substrate performs much better than the conventionally used agar as the germination percentage, shoot and root length, fresh and dry weight as well as the number of leaves are enhanced. The PE-PHs exhibit a higher absorption capacity of the plant growth medium, that is, 36 g·g compared to agar, that is, 20 g·g, and also survive autoclaving conditions without failing. The key advantage over standard agar substrates is that they can be reused several times and also without prior sterilization. These results suggest that PE-PHs with exceptional absorption/retention properties and robustness have great potential as soilless substrates for in vitro plant cultivation.
基于（3-丙烯酰胺丙基）三甲基氯化铵的高度多孔和 3D 相互连接的微细胞聚电解质聚 HIPE（PE-PH）整体作为无土栽培基质在体外胚胎培养中的应用进行了讨论。从鹰嘴豆种子中分离出的胚胎轴接种到整体的表面并允许发芽。发芽研究表明，新披露的 PE-PH 基质的性能优于常规使用的琼脂，因为发芽百分比、芽和根长、鲜重和干重以及叶片数量都得到了提高。PE-PHs 对植物生长介质的吸收能力更高，即 36 g·g 相比之下，琼脂为 20 g·g，并且在不失效的情况下也能承受高压灭菌条件。与标准琼脂基质相比的主要优势是它们可以重复使用多次，也无需事先消毒。这些结果表明，具有特殊吸收/保留性能和坚固性的 PE-PHs 具有作为体外植物培养无土基质的巨大潜力。
