Hunan Provincial Key Laboratory for Synthetic Biology of Traditional Chinese Medicine, School of Pharmaceutical Sciences, Hunan University of Medicine, Huaihua, PR China.
Department of Orthopedics, Xiangya Hospital, Central South University, Changsha, PR China.
Nat Prod Res. 2023 Mar;37(6):912-918. doi: 10.1080/14786419.2022.2096606. Epub 2022 Jul 8.
Benzophenanthridine alkaloids of secondary metabolites from Chinese herb medicine are the excellent anticancer agent to fight sensitive and resistant breast cancer, which is one of the major malignant tumors in females. In the present study, a new benzophenanthridine alkaloid derivatives 8,12-dimethoxysanguinarine (, SG-A) was isolated from . And MCF-7 cell lines were strongly inhibited by SG-A with an IC value of 7.45 μΜ. Furthermore, SG-A strikingly induced non-apoptotic cell death via necroptosis in MCF-7 cells through flow cytometry, Hoechest 33258 and TEM cell morphology analysis. The results suggested that SG-A was found to induce cell necroptosis in MCF-7 cells.
来自中药次生代谢产物的苯并菲啶生物碱是对抗敏感和耐药乳腺癌的优秀抗癌剂，乳腺癌是女性的主要恶性肿瘤之一。在本研究中，从 中分离得到了一种新的苯并菲啶生物碱衍生物 8,12-二甲氧基血根碱（SG-A）。并且，SG-A 对 MCF-7 细胞系具有强烈的抑制作用，IC 值为 7.45 μΜ。此外，通过流式细胞术、Hoechest 33258 和 TEM 细胞形态分析，SG-A 显著诱导 MCF-7 细胞发生非凋亡性细胞死亡即坏死性细胞死亡。结果表明，SG-A 可诱导 MCF-7 细胞发生细胞坏死性死亡。
