National Institute of Standards, Engineering and Surface Metrology Laboratory, Tersa St., El haram, El Giza, Egypt.
J Microsc. 2022 Sep;287(3):156-166. doi: 10.1111/jmi.13133. Epub 2022 Jul 25.
Since the red blood cell shape affects the oxygen transport, so a robust method to reconstruct the 3D shape of an RBC from different projections is presented. A robust one-piece polarizing holographic microscope setup is used to record inline holograms of normal and cancerous red blood cells (RBCs) with high stability. The inline holograms are corrected by flat fielding and windowed Fourier filtering methods to mitigate the zero-order and the defocused twin image due to the inline recording configuration to the least measure. The corrected inline holograms are then reconstructed by the angular spectrum method to extract the 2D wrapping phase-contrast images. The 2D wrapping phase-contrast images are then unwrapped using the graph cuts algorithm to extract the continuous 2D phase-contrast images. The continuous 2D phase-contrast images are reconstructed at different projections by the multiplicative technique to extract the 3D shape of the normal and the cancerous RBCs. Experimental results show that any deformation in the shape of the normal and the cancerous RBCs can be seen clearly at any rotational angle in 3D. This method, which is based on the degree of deformation from the best fitting, can be used as an alternative method of counting method for discrimination between normal and cancerous cells and hence diagnoses the disease easily.
由于红细胞的形状会影响氧气的输送，因此提出了一种从不同投影重建红细胞 3D 形状的稳健方法。使用稳健的单片偏光全息显微镜设置，以高稳定性记录正常和癌细胞（RBC）的直线全息图。通过平面场和窗口傅里叶滤波方法对直线记录配置引起的零阶和离焦孪生像进行校正，以将其校正到最小程度。然后，通过角谱法对校正后的直线全息图进行重建，以提取二维包裹相位对比图像。然后，使用图割算法对二维包裹相位对比图像进行解包裹，以提取连续的二维相位对比图像。通过乘法技术，从不同的投影重建连续的二维相位对比图像，以提取正常和癌细胞的 3D 形状。实验结果表明，在 3D 中，任何正常和癌细胞形状的变形都可以在任何旋转角度清晰地看到。这种方法基于最佳拟合的变形程度，可以用作区分正常和癌细胞的计数方法的替代方法，从而更容易诊断疾病。
