Donnarumma Dario, Rawat Nitin, Brodoline Alexey
Laboratoire Charles Coulomb - UMR 5221 CNRS-Université Montpellier, Place Eugène Bataillon, Montpellier, France.
Microsc Res Tech. 2018 Dec;81(12):1361-1365. doi: 10.1002/jemt.23076. Epub 2018 Nov 15.
A new blood flow imaging (BFI) technique using digital holography with double illumination of the sample is proposed. We imaged the moving red blood cells (RBCs) using a two microscope objective lenses setup. The setup consists in a larger angle of separation (90 °) between the two illumination beams, allowing a wider angular rotation at good z resolution. Moreover, the setup geometry allows an easier displacement of the sample in all directions. Results show that this technique is able to perform phase-shifting reconstruction for the two beams at the same time which is more suitable for the future implementation of live 3D holography. Experimental results are carried out for the verification of the effectiveness of the proposed technique on a zebrafish larvae sample. RESEARCH HIGHLIGHTS: Blood flow imaging techniques are often invasive and image analysis is time consuming. To alleviate this issue an imaging technique based on dual illumination in holographic domain is proposed. This method has been validated on zebrafish embryos.
提出了一种利用数字全息术对样品进行双照明的新型血流成像（BFI）技术。我们使用双显微镜物镜设置对移动的红细胞（RBC）进行成像。该设置包括两个照明光束之间更大的分离角（90°），在良好的z分辨率下允许更宽的角旋转。此外，设置的几何结构允许样品在所有方向上更容易地移动。结果表明，该技术能够同时对两束光进行相移重建，这更适合未来实时3D全息术的实现。对斑马鱼幼虫样本进行了实验结果，以验证所提出技术的有效性。研究亮点：血流成像技术通常具有侵入性，图像分析耗时。为缓解这一问题，提出了一种基于全息域双照明的成像技术。该方法已在斑马鱼胚胎上得到验证。
