Center for Reproductive Medicine, Center for Prenatal Genetics, First Hospital of Jilin University, Changchun, Jilin, China.
PLoS One. 2022 Jul 8;17(7):e0270828. doi: 10.1371/journal.pone.0270828. eCollection 2022.
The association between methionine synthase (MTR) A2756G (rs1805087) polymorphism and the susceptibility to congenital heart disease (CHD) has not been fully determined. A meta-analysis of case-control studies was performed to systematically evaluate the above association. Studies were identified by searching the PubMed, Embase, Web of Science, China National Knowledge Infrastructure, and WanFang databases from inception to June 20, 2021. Two authors independently performed literature search, data extraction, and quality assessment. Predefined subgroup analyses were carried out to evaluate the impact of the population ethnicity, source of healthy controls (community or hospital-based), and methods used for genotyping on the outcomes. A random-effects model was used to combine the results, and 12 studies were included. Results showed that MTR A2756G polymorphism was not associated with CHD susceptibility under the allele model (odds ratio [OR]: 0.96, 95% confidence interval [CI]: 0.86 to 1.07, P = 0.43, I2 = 4%), heterozygote model (OR: 0.95, 95% CI: 0.84 to 1.07, P = 0.41, I2 = 0%), homozygote model (OR: 1.00, 95% CI: 0.64 to 1.55, P = 0.99, I2 = 17%), dominant genetic model (OR: 0.95, 95% CI: 0.84 to 1.07, P = 0.41, I2 = 0%), or recessive genetic model (OR: 0.94, 95% CI: 0.62 to 1.43, P = 0.32, I2 = 13%). Consistent results were found in subgroup analyses between Asian and Caucasian populations in studies with community and hospital-derived controls as well as in studies with PCR-RFLP and direct sequencing (all P values for subgroup differences > 0.05). In conclusion, current evidence does not support an association between MTR A2756G polymorphism and CHD susceptibility.
亚甲基四氢叶酸还原酶（MTR）A2756G（rs1805087）多态性与先天性心脏病（CHD）易感性之间的关系尚未完全确定。进行了一项病例对照研究的荟萃分析，以系统评估上述关联。通过检索 PubMed、Embase、Web of Science、中国国家知识基础设施和万方数据库，从成立到 2021 年 6 月 20 日，确定了研究。两位作者独立进行文献检索、数据提取和质量评估。进行了预设的亚组分析，以评估人群种族、健康对照组的来源（社区或基于医院）以及用于基因分型的方法对结果的影响。使用随机效应模型合并结果，共纳入 12 项研究。结果显示，在等位基因模型下（OR：0.96，95%CI：0.86 至 1.07，P=0.43，I2=4%），MTR A2756G 多态性与 CHD 易感性无关；杂合子模型（OR：0.95，95%CI：0.84 至 1.07，P=0.41，I2=0%）、纯合子模型（OR：1.00，95%CI：0.64 至 1.55，P=0.99，I2=17%）、显性遗传模型（OR：0.95，95%CI：0.84 至 1.07，P=0.41，I2=0%）或隐性遗传模型（OR：0.94，95%CI：0.62 至 1.43，P=0.32，I2=13%）。在基于社区和基于医院的对照组的研究以及基于 PCR-RFLP 和直接测序的研究中，亚洲和高加索人群之间的亚组分析以及研究中均发现了一致的结果（所有亚组差异的 P 值均大于 0.05）。总之，目前的证据不支持 MTR A2756G 多态性与 CHD 易感性之间存在关联。
