Baglin T P, Pollock A
Br J Haematol. 1987 Apr;65(4):387-9. doi: 10.1111/j.1365-2141.1987.tb04137.x.
A method for rosetting neutrophils was developed and optimal rosetting conditions determined. The adhesive properties of neutrophils and their ability to form rosettes were studied for cells in the circulating pool, and following exercise when the circulating pool had been enriched by neutrophils from the marginated pool. We found the marginated pool to be the same as the circulating pool in terms of rosetting subpopulations and the in vitro adhesive properties of these subpopulations, indicating that the two pools can be considered kinetically as one. We also showed that non-rosetting neutrophils can develop the ability to form rosettes following adhesion to glass in the presence of autologous serum.
开发了一种嗜中性粒细胞玫瑰花结形成方法，并确定了最佳玫瑰花结形成条件。研究了循环池中细胞以及运动后循环池因边缘池嗜中性粒细胞增多而富含细胞时，嗜中性粒细胞的黏附特性及其形成玫瑰花结的能力。我们发现，就玫瑰花结形成亚群及其体外黏附特性而言，边缘池与循环池相同，这表明这两个池在动力学上可被视为一个整体。我们还表明，非玫瑰花结形成嗜中性粒细胞在自体血清存在下黏附于玻璃后可获得形成玫瑰花结的能力。
