Szczepanik A, Klimas R, Wysocki H
J Immunol Methods. 1986 May 1;89(1):49-51. doi: 10.1016/0022-1759(86)90030-x.
A simple method for the evaluation of rosetting polymorphonuclear neutrophils is described. It uses small (200 microliter) quantities of heparinized whole blood and is simpler than the micromethod proposed by Buescher and Gallin. The high concentration of neutrophils and paucity of lymphocytes considerably simplify the evaluation of specimens and make the technique particularly useful in granulopenic states. There was a close correlation between results obtained using this technique and those from a standard method requiring the isolation of polymorphonuclear neutrophils.
本文描述了一种评估玫瑰花结形成的多形核中性粒细胞的简单方法。该方法使用少量（200微升）肝素化全血，比Buescher和Gallin提出的微量法更简单。中性粒细胞浓度高而淋巴细胞数量少，大大简化了标本评估，使该技术在粒细胞减少状态下特别有用。使用该技术获得的结果与需要分离多形核中性粒细胞的标准方法的结果之间存在密切相关性。
