College of Food Science, Southwest University, Chongqing 400715, China.
College of Food Science, Southwest University, Chongqing 400715, China.
Food Chem. 2022 Nov 30;395:133622. doi: 10.1016/j.foodchem.2022.133622. Epub 2022 Jul 2.
This study aims to produce oligosaccharides by microwave-assisted hydrothermal hydrolysis of agarose, which is a more environmentally friendly and green economical approach than acid or enzymatic hydrolysis. Under the optimized condition of 160 °C and 25 min, total liquefaction of agarose was achieved, the dominant products in the hydrolysate are agaro-oligosaccharides (AOs), with only a small portion of d-galactose and 5- hydroxymethylfurfural (5-HMF). This approach can produce even and odd number AOs simultaneously, which is due to the random hydrolysis of α-1,3 glycosidic linkage at first and subsequent hydrolysis of the β-1,4 glycosidic linkage at the reducing end. The yield of AOs of a low degree of polymerization (DP) can reach about 57% of the theoretical maximum, while the total yield of AOs is over 90%. In conclusion, microwave-assisted hydrothermal hydrolysis is a way of producing oligosaccharides from agarose with extra-high efficiency and practical significance.
本研究旨在通过微波辅助水热解琼脂糖来生产低聚糖，这是一种比酸解或酶解更环保、更经济的方法。在 160°C 和 25 分钟的优化条件下，琼脂糖完全液化，水解产物中的主要产物是琼脂低聚糖（AOs），只有一小部分半乳糖和 5-羟甲基糠醛（5-HMF）。这种方法可以同时产生偶数和奇数个 AOs，这是由于首先随机水解α-1,3 糖苷键，然后在还原端水解β-1,4 糖苷键。低聚合度（DP）AOs 的产率可达理论最大值的约 57%，而 AOs 的总产率超过 90%。总之，微波辅助水热解是一种从琼脂糖中高效生产具有实际意义的低聚糖的方法。
