College of Food Science, Southwest University, Chongqing 400715, China; Guangxi Key Lab of Agricultural Resources Chemistry and Biotechnology, College of Chemistry and Food Science, Yulin Normal University, Yulin, Guangxi 537000, PR China.
College of Food Science, Southwest University, Chongqing 400715, China.
Int J Biol Macromol. 2023 Nov 1;251:126319. doi: 10.1016/j.ijbiomac.2023.126319. Epub 2023 Aug 14.
Hydrolysis of agar or agarose can yield two types of oligosaccharides: agaro-oligosaccharides (AOS) and neoagaro-oligosaccharides (NAOS). These oligosaccharides have various biological activities and promising applications in the future food industry and pharmaceuticals. In this study, we prepared AOS from agarose by microwave-assisted hydrothermal hydrolysis and then used a commercial β-galactosidase to treat AOS for producing NAOS. A complete conversion from agarose to AOS or NAOS can be achieved by microwave hydrothermal treatment and one-step enzyme reaction, and the production process was completely green. In addition, we combined β-galactosidase and α-neoagarobiose hydrolase from Saccharophagus degradans 2-40 (SdNABH) to treat AOS, and AOS was completely converted into monosaccharides. Then the results of the inhibitory activity of AOS on the growth of Streptococcus mutans showed that AOS might be a good potential sugar substitute for dental caries prevention. This study provides an efficient approach for the production of multiple mixed degrees of polymerization (DP) of pure AOS and NAOS without requiring acid catalyst and agarases while simplifying the production processes and reducing costs.
琼脂低聚糖（AOS）和新琼胶低聚糖（NAOS）。这些低聚糖具有多种生物活性，在未来的食品工业和制药领域有广阔的应用前景。本研究采用微波辅助水热水解法从琼胶中制备 AOS，然后用商业β-半乳糖苷酶处理 AOS 以生产 NAOS。通过微波水热处理和一步酶反应，可以完全将琼脂转化为 AOS 或 NAOS，生产过程完全绿色环保。此外，我们将来自 Saccharophagus degradans 2-40 的β-半乳糖苷酶和α-新琼二糖水解酶（SdNABH）组合起来处理 AOS，AOS 可完全转化为单糖。然后，AOS 对变形链球菌生长抑制活性的结果表明，AOS 可能是一种预防龋齿的潜在良好的糖替代品。本研究提供了一种高效的方法，可在不使用酸催化剂和琼脂酶的情况下生产具有多种聚合度（DP）的纯 AOS 和 NAOS，同时简化了生产工艺并降低了成本。
