Johnston N C, Goldfine H, Malthaner M, Seelig J
Biochim Biophys Acta. 1987 May 29;899(2):302-6. doi: 10.1016/0005-2736(87)90412-3.
Palmitic acid specifically deuterated at different carbon atoms, has been incorporated biosynthetically into the membrane lipids of Clostridium butyricum. The lipids of this organism are rich in plasmalogens and their glycerol acetals and exhibit an unusual fatty acyl and alkenyl chain distribution with saturated chains mainly at the sn-2 position and unsaturated chains at the sn-1 position. The ordering of the deuterated hydrocarbon chains in whole cells was measured with deuterium nuclear magnetic resonance and was compared to the order profiles of isolated cell membranes and membranes formed from the total phospholipid extract. The shape of the order profiles was similar for all three membranes, but the absolute values of the order profiles in whole cells and isolated membranes were lower than those of the liposomal lipids. The order profiles have the same characteristic shape as those found for the lamellar liquid-crystalline phases of synthetic diacylphospholipids.
在不同碳原子上进行特异性氘代的棕榈酸已通过生物合成方式掺入丁酸梭菌的膜脂中。该生物体的脂质富含缩醛磷脂及其甘油缩醛，并且呈现出不寻常的脂肪酰基和烯基链分布，饱和链主要位于sn-2位，不饱和链位于sn-1位。用氘核磁共振测量了全细胞中氘代烃链的有序性，并将其与分离的细胞膜和由总磷脂提取物形成的膜的有序性图谱进行了比较。这三种膜的有序性图谱形状相似，但全细胞和分离膜中有序性图谱的绝对值低于脂质体脂质的绝对值。这些有序性图谱具有与合成二酰基磷脂的层状液晶相所发现的相同特征形状。
