Institute for Bioengineering, School of Engineering, University of Edinburgh, King's Buildings, Edinburgh, EH9 3BF, UK; UK Centre for Mammalian Synthetic Biology, University of Edinburgh, King's Buildings, Edinburgh, EH9 3BD, UK.
Institute for Bioengineering, School of Engineering, University of Edinburgh, King's Buildings, Edinburgh, EH9 3BF, UK; UK Centre for Mammalian Synthetic Biology, University of Edinburgh, King's Buildings, Edinburgh, EH9 3BD, UK.
Trends Biotechnol. 2022 Nov;40(11):1299-1312. doi: 10.1016/j.tibtech.2022.06.001. Epub 2022 Jul 5.
Synthetic biology is moving towards bioengineering multicellular mammalian systems that are poised to advance tissue engineering, biomedicine, and the food industry. Despite progress, the field lacks a framework of standards that could greatly accelerate further development. Here, we explore the landscape of standards for multicellular mammalian synthetic biology. We discuss the limits of current technical standards and categorise unaddressed parameters into an abstraction hierarchy. We then define the concept of a 'synthetic multicellular mammalian system' and apply our standard hierarchy framework to illustrate how it could aid bioengineering endeavours. We conclude with promising areas that could shape the future of the field, flagging the need for a critical and holistic consideration of standards that requires cross-disciplinary dialogue.
合成生物学正向工程化多细胞哺乳动物系统迈进，这有望推动组织工程、生物医学和食品工业的发展。尽管取得了进展，但该领域缺乏一个能够极大加速进一步发展的标准框架。在这里，我们探讨了多细胞哺乳动物合成生物学标准的现状。我们讨论了当前技术标准的局限性，并将未解决的参数分类为抽象层次结构。然后，我们定义了“合成多细胞哺乳动物系统”的概念，并应用我们的标准层次结构框架来说明它如何有助于生物工程的努力。最后，我们总结了有前途的领域，这些领域可能塑造该领域的未来，同时也需要对标准进行批判性和全面的考虑，这需要跨学科的对话。
