Mott G E, Jackson E M, Prihoda T J, McMahan C A
Biochim Biophys Acta. 1987 Jun 2;919(2):190-8. doi: 10.1016/0005-2760(87)90207-4.
We analyzed the effects of dietary cholesterol, type of dietary fat, sex and sire progeny family on lecithin-cholesterol acyltransferase activity in 80 adult baboons. The animals were the progeny of 80 dams and 6 sires and were randomly assigned at birth to breast feeding or to one of three formulas containing 0.02, 0.30 or 0.60 mg cholesterol/ml. After weaning at 4 months of age the animals were fed one of four diets that were either high or low in cholesterol with 40% of the calories from either saturated or unsaturated fat. The fractional and molar rates of lecithin-cholesterol acyltransferase activity were measured at 7-8 years of age by an HPLC method. Infant diet (breast vs. formula feeding or level of cholesterol in formula had no effect on enzyme activity later in life. The adult diets that were high in cholesterol decreased the fractional lecithin-cholesterol acyltransferase rate by 20% / compared to diets low in cholesterol (7.89 vs. 9.84%/h, P less than 0.002), but dietary cholesterol did not affect the molar activity. Animals fed the high cholesterol diets had higher unesterified cholesterol concentrations compared to those fed the low cholesterol diets (38.1 mg/dl vs. 31.6 mg/dl, P less than 0.0001). The molar lecithin-cholesterol acyltransferase rate was increased 13% by saturated compared to unsaturated fat (83.3 vs. 73.6 nmol/h per ml plasma, P less than 0.07), but no effect of dietary fat was observed on the fractional enzyme activity. Females compared to males had significantly higher fractional (10.9 vs. 7.14%/h, P less than 0.0001) and molar lecithin-cholesterol acyltransferase activities (99.3 vs. 61.7 nmol/h per ml plasma, P less than 0.0001). After adjustment for the effects of diet and sex we observed differences in the fractional activity (range, 7.2-10.8%/h, P less than 0.04) and in the molar rate (range, 63.6-99.8 nmol/h per ml plasma, P less than 0.07) among the six sire progeny groups. The differences among sire progeny groups are evidence for genetic differences in lecithin-cholesterol acyltransferase activities among the baboon families.
我们分析了膳食胆固醇、膳食脂肪类型、性别和父系后代家族对80只成年狒狒卵磷脂胆固醇酰基转移酶活性的影响。这些动物是80只母狒狒和6只公狒狒的后代，出生时被随机分配进行母乳喂养或采用三种配方奶之一喂养，三种配方奶中胆固醇含量分别为0.02、0.30或0.60毫克/毫升。4个月龄断奶后，这些动物被喂食四种饮食中的一种，这些饮食胆固醇含量有高有低，且40%的热量来自饱和脂肪或不饱和脂肪。在7至8岁时，通过高效液相色谱法测量卵磷脂胆固醇酰基转移酶活性的分数率和摩尔率。婴儿期饮食（母乳喂养与配方奶喂养或配方奶中的胆固醇水平）对其成年后的酶活性没有影响。与低胆固醇饮食相比，高胆固醇的成年饮食使卵磷脂胆固醇酰基转移酶的分数率降低了20%（分别为7.89%/小时和9.84%/小时，P<0.002），但膳食胆固醇不影响摩尔活性。与喂食低胆固醇饮食的动物相比，喂食高胆固醇饮食的动物未酯化胆固醇浓度更高（分别为38.1毫克/分升和31.6毫克/分升，P<0.0001）。与不饱和脂肪相比，饱和脂肪使卵磷脂胆固醇酰基转移酶的摩尔率提高了13%（分别为每毫升血浆83.3纳摩尔/小时和73.6纳摩尔/小时，P<0.07），但未观察到膳食脂肪对分数酶活性有影响。与雄性相比，雌性的卵磷脂胆固醇酰基转移酶分数活性（分别为10.9%/小时和7.14%/小时，P<0.0001）和摩尔活性（分别为每毫升血浆99.3纳摩尔/小时和61.7纳摩尔/小时，P<0.0001）显著更高。在调整饮食和性别的影响后，我们观察到六个父系后代组之间在分数活性（范围为7.2 - 10.8%/小时，P<0.04）和摩尔率（范围为每毫升血浆63.6 - 99.8纳摩尔/小时，P<0.07）上存在差异。父系后代组之间的差异证明了狒狒家族中卵磷脂胆固醇酰基转移酶活性存在遗传差异。
