• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

评价两种嵌合 O 型口蹄疫病毒疫苗在猪和牛中的免疫原性和交叉反应性。

Evaluation of immunogenicity and cross-reactive responses of  vaccines prepared from two chimeric serotype O foot-and-mouth disease viruses in pigs and cattle.

机构信息

State Key Laboratory of Veterinary Etiological Biology, OIE/National Foot-and-Mouth Disease Reference Laboratory of China, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, China.

出版信息

Vet Res. 2022 Jul 8;53(1):56. doi: 10.1186/s13567-022-01072-7.

DOI:10.1186/s13567-022-01072-7
PMID:35804412
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9270804/
Abstract

Foot-and-mouth disease (FMD) remains a very serious barrier to agricultural development and the international trade of animals and animal products. Recently, serotype O has been the most prevalent FMDV serotype in China, and it has evolved into four different lineages: O/SEA/Mya-98, O/ME-SA/PanAsia, O/ME-SA/Ind-2001 and O/Cathay. PanAsia-2, belonging to the O/ME-SA topotype, is prevalent in neighbouring countries and poses the risk of cross-border spread in China. This study aimed to develop a promising vaccine candidate strain that can not only provide the best protection against all serotype O FMDVs circulating in China but also be used as an emergency vaccine for the prevention and control of transboundary incursion of PanAsia-2. Here, two chimeric FMDVs (rHN/TURVP1 and rHN/NXVP1) featuring substitution of VP1 genes of the O/TUR/5/2009 vaccine strain (PanAsia-2) and O/NXYCh/CHA/2018 epidemic strain (Mya98) were constructed and evaluated. The biological properties of the two chimeric FMDVs were similar to those of the wild-type (wt) virus despite slight differences in plaque sizes observed in BHK-21 cells. The structural protein-specific antibody titres induced by the rHN/TURVP1 and wt virus vaccines in pigs and cows were higher than those induced by the rHN/NXVP1 vaccine at 28-56 dpv. The vaccines prepared from the two chimeric viruses and wt virus all induced the production of protective cross-neutralizing antibodies against the viruses of the Mya-98, PanAsia and Ind-2001 lineages in pigs and cattle at 28 dpv; however, only the animals vaccinated with the rHN/TURVP1 vaccine produced a protective immune response to the field isolate of the Cathay lineage at 28 dpv, whereas the animals receiving the wt virus and the rHN/NXVP1 vaccines did not, although the wt virus and O/GXCX/CHA/2018 both belong to the Cathay topotype. This study will provide very useful information to help develop a potential vaccine candidate for the prevention and control of serotype O FMD in China.

摘要

口蹄疫（FMD）仍然是农业发展和动物及动物产品国际贸易的一个非常严重的障碍。最近，在中国流行的血清型 O 是最主要的 FMDV 血清型，它已经进化成四个不同的谱系：O/SEA/Mya-98、O/ME-SA/PanAsia、O/ME-SA/Ind-2001 和 O/Cathay。属于 O/ME-SA 原型的 PanAsia-2 在邻国流行，在中国存在跨境传播的风险。本研究旨在开发一种有前途的疫苗候选株，不仅能为中国流行的所有 O 型 FMDV 提供最佳保护，还能用作预防和控制 PanAsia-2 跨界入侵的应急疫苗。在此，构建并评估了两个嵌合 FMDV（rHN/TURVP1 和 rHN/NXVP1），其特征是替换 O/TUR/5/2009 疫苗株（PanAsia-2）和 O/NXYCh/CHA/2018 流行株（Mya98）的 VP1 基因。尽管在 BHK-21 细胞中观察到噬斑大小略有差异，但两个嵌合 FMDV 的生物学特性与野生型（wt）病毒相似。在猪和牛中，rHN/TURVP1 和 wt 病毒疫苗诱导的结构蛋白特异性抗体滴度在 28-56dpv 时均高于 rHN/NXVP1 疫苗诱导的抗体滴度。由两个嵌合病毒和 wt 病毒制备的疫苗均能在 28dpv 时诱导猪和牛对 Mya-98、PanAsia 和 Ind-2001 谱系病毒产生保护性中和抗体；然而，只有接种 rHN/TURVP1 疫苗的动物在 28dpv 时对田间分离的 Cathay 谱系产生了保护性免疫应答，而接种 wt 病毒和 rHN/NXVP1 疫苗的动物则没有，尽管 wt 病毒和 O/GXCX/CHA/2018 均属于 Cathay 原型。本研究将为中国 O 型 FMD 的预防和控制提供有价值的信息，帮助开发潜在的疫苗候选株。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/41cd/9270804/b0bcf22208a8/13567_2022_1072_Fig9_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/41cd/9270804/467b2e296d7c/13567_2022_1072_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/41cd/9270804/28cb6091f933/13567_2022_1072_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/41cd/9270804/3b267f329dda/13567_2022_1072_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/41cd/9270804/27aa37e4a3ff/13567_2022_1072_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/41cd/9270804/cec439ecafb3/13567_2022_1072_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/41cd/9270804/ba4633ee9973/13567_2022_1072_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/41cd/9270804/3d05d4d164a4/13567_2022_1072_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/41cd/9270804/9760a1d25baf/13567_2022_1072_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/41cd/9270804/b0bcf22208a8/13567_2022_1072_Fig9_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/41cd/9270804/467b2e296d7c/13567_2022_1072_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/41cd/9270804/28cb6091f933/13567_2022_1072_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/41cd/9270804/3b267f329dda/13567_2022_1072_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/41cd/9270804/27aa37e4a3ff/13567_2022_1072_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/41cd/9270804/cec439ecafb3/13567_2022_1072_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/41cd/9270804/ba4633ee9973/13567_2022_1072_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/41cd/9270804/3d05d4d164a4/13567_2022_1072_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/41cd/9270804/9760a1d25baf/13567_2022_1072_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/41cd/9270804/b0bcf22208a8/13567_2022_1072_Fig9_HTML.jpg

相似文献

1
Evaluation of immunogenicity and cross-reactive responses of  vaccines prepared from two chimeric serotype O foot-and-mouth disease viruses in pigs and cattle.评价两种嵌合 O 型口蹄疫病毒疫苗在猪和牛中的免疫原性和交叉反应性。
Vet Res. 2022 Jul 8;53(1):56. doi: 10.1186/s13567-022-01072-7.
2
Molecular Basis of Antigenic Drift in Serotype O Foot-and-Mouth Disease Viruses (2013-2018) from Southeast Asia.东南亚 2013-2018 年 O 型口蹄疫病毒抗原漂移的分子基础。
Viruses. 2021 Sep 21;13(9):1886. doi: 10.3390/v13091886.
3
Vaccine strain of O/ME-SA/Ind-2001e of foot-and-mouth disease virus provides high immunogenicity and broad antigenic coverage.口蹄疫病毒 O/ME-SA/Ind-2001e 疫苗株具有较高的免疫原性和广泛的抗原覆盖范围。
Antiviral Res. 2020 Oct;182:104920. doi: 10.1016/j.antiviral.2020.104920. Epub 2020 Aug 20.
4
Selection of vaccine strains for serotype O foot-and-mouth disease viruses (2007-2012) circulating in Southeast Asia, East Asia and Far East.东南亚、东亚和远东地区流行的 O 型口蹄疫病毒（2007-2012 年）疫苗株的选择。
Vaccine. 2017 Dec 18;35(51):7147-7153. doi: 10.1016/j.vaccine.2017.10.099. Epub 2017 Nov 20.
5
Improved foot-and-mouth disease vaccine with O PanAsia-2 strain protect pigs against O/Jincheon/SKR/2014 originated from South Korea.改进的 O PanAsia-2 株口蹄疫疫苗可预防源自韩国的 O/Jincheon/SKR/2014 对猪的感染。
Vaccine. 2020 Jan 29;38(5):1120-1128. doi: 10.1016/j.vaccine.2019.11.029. Epub 2019 Dec 3.
6
Cross-protective efficacy of engineering serotype A foot-and-mouth disease virus vaccine against the two pandemic strains in swine.工程化A型口蹄疫病毒疫苗对猪体内两种大流行毒株的交叉保护效力
Vaccine. 2015 Oct 26;33(43):5772-5778. doi: 10.1016/j.vaccine.2015.09.055. Epub 2015 Oct 1.
7
Genetic and antigenic characterization of serotype O FMD viruses from East Africa for the selection of suitable vaccine strain.东非口蹄疫病毒血清型 O 的遗传和抗原特征分析，以选择合适的疫苗株。
Vaccine. 2017 Dec 14;35(49 Pt B):6842-6849. doi: 10.1016/j.vaccine.2017.10.040.
8
Improved foot-and-mouth disease vaccine, O TWN-R, protects pigs against SEA topotype virus occurred in South Korea.改进型口蹄疫疫苗 O TWN-R 可保护猪免受在韩国发生的 SEA 拓扑型病毒的侵害。
Vet Microbiol. 2019 Sep;236:108374. doi: 10.1016/j.vetmic.2019.07.026. Epub 2019 Jul 25.
9
Broad immunogenic spectrum of monovalent and trivalent foot-and-mouth disease virus vaccines containing O campos, A24 cruzeiro and A Argentina 2001 strains against circulating viral lineages in cattle and pigs.单价和三价口蹄疫病毒疫苗对流行于牛和猪的病毒谱系具有广泛的免疫原性，这些疫苗包含 O Campos、A24 Cruzeiro 和 A Argentina 2001 株。
Vaccine. 2023 Sep 7;41(39):5782-5790. doi: 10.1016/j.vaccine.2023.08.007. Epub 2023 Aug 12.
10
Evaluation of a genetically modified foot-and-mouth disease virus vaccine candidate generated by reverse genetics.基于反向遗传学技术构建的口蹄疫病毒基因工程疫苗候选株的评价。
BMC Vet Res. 2012 May 16;8:57. doi: 10.1186/1746-6148-8-57.

引用本文的文献

1
Foot-and-mouth disease virus vaccine with VP1 G-H loop substitution of the Cathay strain broadens antigen spectrum.具有中国株VP1 G-H环替换的口蹄疫病毒疫苗拓宽了抗原谱。
Appl Microbiol Biotechnol. 2025 Sep 11;109(1):199. doi: 10.1007/s00253-025-13561-8.
2
Establishment and Implementation of the Point-of-Care RT-RAA-CRISPR/Cas13a Diagnostic Test for Foot-And-Mouth Disease Virus Serotype O in Pigs.猪口蹄疫病毒O型即时RT-RAA-CRISPR/Cas13a诊断检测方法的建立与应用
Viruses. 2025 May 17;17(5):721. doi: 10.3390/v17050721.
3
Foot-and-mouth disease: genomic and proteomic structure, antigenic sites, serotype relationships, immune evasion, recent vaccine development strategies, and future perspectives.

本文引用的文献

1
Emergency FMD Serotype O Vaccines Protect Cattle against Heterologous Challenge with a Variant Foot-and-Mouth Disease Virus from the O/ME-SA/Ind2001 Lineage.紧急口蹄疫O型疫苗可保护牛免受O/ME-SA/Ind2001谱系变异口蹄疫病毒的异源攻击。
Vaccines (Basel). 2021 Sep 29;9(10):1110. doi: 10.3390/vaccines9101110.
2
A Vaccine Strain of the A/ASIA/Sea-97 Lineage of Foot-and-Mouth Disease Virus with a Single Amino Acid Substitution in the P1 Region That Is Adapted to Suspension Culture Provides High Immunogenicity.一种口蹄疫病毒A/亚洲/Sea-97谱系的疫苗株，其P1区域有一个氨基酸替换，适应悬浮培养，具有高免疫原性。
Vaccines (Basel). 2021 Mar 24;9(4):308. doi: 10.3390/vaccines9040308.
3
口蹄疫：基因组和蛋白质组结构、抗原位点、血清型关系、免疫逃逸、近期疫苗开发策略及未来展望
Vet Res. 2025 Apr 7;56(1):78. doi: 10.1186/s13567-025-01485-0.
4
Foot-and-mouth disease in Asia.亚洲的口蹄疫
Virus Res. 2025 Jan;351:199514. doi: 10.1016/j.virusres.2024.199514. Epub 2024 Dec 19.
5
Virulence and Immune Evasion Strategies of FMDV: Implications for Vaccine Design.口蹄疫病毒的毒力与免疫逃逸策略：对疫苗设计的启示
Vaccines (Basel). 2024 Sep 19;12(9):1071. doi: 10.3390/vaccines12091071.
6
A meta-analysis on the potency of foot-and-mouth disease vaccines in different animal models.口蹄疫疫苗在不同动物模型中的效力的荟萃分析。
Sci Rep. 2024 Apr 18;14(1):8931. doi: 10.1038/s41598-024-59755-4.
7
Molecular evolution, diversity, and adaptation of foot-and-mouth disease virus serotype O in Asia.亚洲口蹄疫病毒O型的分子进化、多样性及适应性
Front Microbiol. 2023 Mar 9;14:1147652. doi: 10.3389/fmicb.2023.1147652. eCollection 2023.
8
Evaluation of Vaccine Strains Developed for Efficient, Broad-Range Protection against Foot-and-Mouth Disease Type O.针对O型口蹄疫开发的高效、广谱保护性疫苗株的评估。
Vaccines (Basel). 2023 Jan 27;11(2):271. doi: 10.3390/vaccines11020271.
Improved foot-and-mouth disease vaccine with O PanAsia-2 strain protect pigs against O/Jincheon/SKR/2014 originated from South Korea.
改进的 O PanAsia-2 株口蹄疫疫苗可预防源自韩国的 O/Jincheon/SKR/2014 对猪的感染。
Vaccine. 2020 Jan 29;38(5):1120-1128. doi: 10.1016/j.vaccine.2019.11.029. Epub 2019 Dec 3.
4
FMD vaccine matching: Inter laboratory study for improved understanding of r values.口蹄疫疫苗匹配：提高 r 值理解的实验室间研究。
J Virol Methods. 2020 Feb;276:113786. doi: 10.1016/j.jviromet.2019.113786. Epub 2019 Nov 22.
5
The VP1 G-H loop hypervariable epitope contributes to protective immunity against Foot and Mouth Disease Virus in swine.VP1 G-H 环高变区表位有助于猪抵抗口蹄疫病毒的保护性免疫。
Vaccine. 2019 Jun 6;37(26):3435-3442. doi: 10.1016/j.vaccine.2019.05.019. Epub 2019 May 10.
6
Chimeric vaccine strain of type O foot-and-mouth disease elicits a strong immune response in pigs against ME-SA and SEA topotypes.O型口蹄疫嵌合疫苗株在猪体内引发针对中东-南非亚拓扑型和东南亚亚拓扑型的强烈免疫反应。
Vet Microbiol. 2019 Feb;229:124-129. doi: 10.1016/j.vetmic.2018.12.023. Epub 2018 Dec 26.
7
Chimeric O1K foot-and-mouth disease virus with SAT2 outer capsid as an FMD vaccine candidate.嵌合 O1K 口蹄疫病毒，以 SAT2 外壳作为口蹄疫疫苗候选物。
Sci Rep. 2018 Sep 12;8(1):13654. doi: 10.1038/s41598-018-31856-x.
8
Foot and mouth disease vaccine strain selection: current approaches and future perspectives.口蹄疫疫苗株的选择：当前方法和未来展望。
Expert Rev Vaccines. 2018 Jul;17(7):577-591. doi: 10.1080/14760584.2018.1492378. Epub 2018 Jul 26.
9
First detection of foot-and-mouth disease virus O/ME-SA/Ind2001 in China.中国首次检出口蹄疫病毒 O/ME-SA/Ind2001。
Transbound Emerg Dis. 2018 Dec;65(6):2027-2031. doi: 10.1111/tbed.12895. Epub 2018 May 9.
10
Selection of vaccine strains for serotype O foot-and-mouth disease viruses (2007-2012) circulating in Southeast Asia, East Asia and Far East.东南亚、东亚和远东地区流行的 O 型口蹄疫病毒（2007-2012 年）疫苗株的选择。
Vaccine. 2017 Dec 18;35(51):7147-7153. doi: 10.1016/j.vaccine.2017.10.099. Epub 2017 Nov 20.