Wang Qian, Liu Qian, Ma Wenxiu, Wang Rui, Li Shuo, Dong Zhongdian, Shao Changwei
Guangdong South China Sea Key Laboratory of Aquaculture for Aquatic Economic Animals, Fisheries College, Guangdong Ocean University, Zhanjiang 524000, China.
Laboratory for Marine Fisheries Science and Food Production Processes, Pilot National Laboratory for Marine Science and Technology, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences (CAFS), Qingdao 266071, China.
Animals (Basel). 2022 Jun 21;12(13):1603. doi: 10.3390/ani12131603.
The eukaryotic translation elongation factor 1 alpha () gene has a well-defined role in protein synthesis. However, its role in external temperature perception and internal sex differentiation and development is still unclear. In this study, was identified and functionally analyzed in Chinese tongue sole (). The cDNA, 1809 bp in length, had a 1386 bp open reading frame (ORF) that encoded a 461 amino acid polypeptide containing one EF-1_alpha domain. expression levels were investigated across different tissues and during gonadal development. In the gonad, showed a sexually dimorphic expression pattern with a statistically higher expression level in the ovary than in the testis from 6 months postfertilization to 3 years postfertilization. Under high temperature (28 °C) treatment during sex differentiation (from 30 days postfertilization to 3 months postfertilization), was statistically down-regulated in males, while the difference was not detected in females. In addition, the dual-luciferase assay exhibited that can respond to high temperature rapidly. Based on these results, might have a dual role in the perception of external temperature changes and sex differentiation regulation.
真核生物翻译延伸因子1α（）基因在蛋白质合成中具有明确的作用。然而，其在外部温度感知以及内部性别分化和发育中的作用仍不清楚。在本研究中，在中国舌鳎（）中鉴定并对其进行了功能分析。该cDNA长度为1809 bp，有一个1386 bp的开放阅读框（ORF），编码一个含有一个EF-1_α结构域的461个氨基酸的多肽。研究了该基因在不同组织以及性腺发育过程中的表达水平。在性腺中，从受精后6个月到受精后3年，该基因呈现出性别二态性表达模式，在卵巢中的表达水平在统计学上高于睾丸。在性别分化期间（从受精后30天到受精后3个月）进行高温（28°C）处理时，雄性中的该基因在统计学上被下调，而在雌性中未检测到差异。此外，双荧光素酶测定表明该基因可快速响应高温。基于这些结果，该基因可能在外部温度变化感知和性别分化调控中具有双重作用。
