Thanh Lam Phuoc, Jiang Qianming, Wichasit Nithat, Batistel Fernanda, Parys Claudia, Guyader Jessie, Loor Juan J
Department of Animal Sciences, Can Tho University, Ninh Kieu, Can Tho 94000, Vietnam.
Department of Animal Sciences and Division of Nutritional Sciences, University of Illinois, Urbana, IL 61801, USA.
Animals (Basel). 2022 Jun 26;12(13):1641. doi: 10.3390/ani12131641.
This study aimed to evaluate the effect of feeding ethyl cellulose rumen-protected methionine (RPM) on skeletal muscle mRNA abundance during the periparturient period. Sixty multiparous Holstein cows were used in a block design and assigned to either a control or RPM diet. The RPM was supplied from −28 to 60 days in milk (DIM) at a rate of 0.09% (prepartum) or 0.10% (postpartum) of dry matter (DM), ensuring a Lys:Met in the metabolizable protein of ~2.8:1. Muscle biopsies were collected at −21, 1, and 21 DIM. Thirty-five target genes associated with nutrient metabolism and biochemical pathways were measured via RT-qPCR. The mRNA abundance of genes associated with amino acid (AA) transport (SLC7A8, SLC43A2), carnitine transport (SLC22A5), insulin signaling (IRS1), and antioxidant response (NFE2L2) had diet × time effect (p < 0.05) due to greater abundance in RPM versus CON cows, especially at 1 and 21 DIM. Members of the AA transport (SLC7A8, SLC25A29, SCL38A9), fatty acid β-oxidation (ACADVL), vitamin transport (SLC5A6, SLC19A2), mTOR pathway (AKT1 and mTOR), antioxidant response (KEAP1, CUL3), CDP-Choline pathway and arginine metabolism had overall greater abundance (p < 0.05) in RPM versus CON cows. Overall, data indicate that RPM can alter nutrient metabolism in the skeletal muscle around parturition partly through alterations in mRNA abundance.
本研究旨在评估围产期饲喂瘤胃保护性蛋氨酸（RPM）对骨骼肌mRNA丰度的影响。采用区组设计，选用60头经产荷斯坦奶牛，分为对照组和RPM日粮组。在产奶量为−28至60天期间供应RPM，干物质（DM）的供应比例为0.09%（产前）或0.10%（产后），确保可代谢蛋白质中的赖氨酸:蛋氨酸约为2.8:1。在产奶量−21、1和21天时采集肌肉活检样本。通过RT-qPCR检测35个与营养代谢和生化途径相关的靶基因。与氨基酸（AA）转运（SLC7A8、SLC43A2）、肉碱转运（SLC22A5）、胰岛素信号传导（IRS1）和抗氧化反应（NFE2L2）相关的基因的mRNA丰度存在日粮×时间效应（p<0.05），因为与对照组奶牛相比，RPM组奶牛的丰度更高，尤其是在产奶量1和21天时。AA转运（SLC7A8、SLC25A29、SCL38A9）、脂肪酸β-氧化（ACADVL）、维生素转运（SLC5A6、SLC19A2）、mTOR途径（AKT1和mTOR）、抗氧化反应（KEAP1、CUL3）、CDP-胆碱途径和精氨酸代谢的成员在RPM组奶牛中的总体丰度更高（p<0.05）。总体而言，数据表明RPM可以部分通过改变mRNA丰度来改变分娩前后骨骼肌中的营养代谢。
