Zhou Luli, Li Hui, Hou Guanyu, Wang Jian, Zhou Hanlin, Wang Dingfa
Tropical Crops Genetic Resources Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, China.
College of Animal Science and Technology, Hainan University, Haikou 570228, China.
Animals (Basel). 2022 Jun 28;12(13):1661. doi: 10.3390/ani12131661.
This study investigates the effects of vine tea (Ampelopsis grossedentata) extract (AGE) on meat quality, gut microbiota and cecal content metabolites of Wenchang broilers. A total of 240 female Wenchang broilers aged 70 days were randomly allocated into four groups with five replicates of twelve broilers each. Broilers were fed a corn-soybean basal diet supplemented with AGE at 0 (T1), 0.2% (T2), 0.4% (T3) and 0.6% (T4) until 124 days of age. The whole feeding trial lasted 54 days. Results suggest that the content of total triglycerides and low-density lipoprotein cholesterol in serum of broilers are linearly reduced with dietary AGE supplementation (p < 0.05). The T3 and T4 groups had higher (p < 0.05) a* value in thigh and breast muscles than the T1 group. Additionally, the dietary supplementation of AGE decreased the shear force and drip loss of both thigh and breast muscles linearly (p < 0.05). Compared with the T1 group, AGE supplementation increased the levels of inosine monophosphate (IMP) significantly (p < 0.05) in both the thigh and breast muscles. Furthermore, an increase (p < 0.05) in the total unsaturated fatty acid (USFA), polyunsaturated fatty acids (PUFA) and the ratio of unsaturated fatty acids to saturated fatty acid (USFA: SFA) in both the thigh and breast muscles in the T3 group was observed. Higher abundance of Bacteroidota (p < 0.05) and lower abundance of Firmicutes (p < 0.05) were observed in the T3 group. The abundance of Faecalibacterium was significantly decreased (p < 0.05) in the T3 group compared with the T1 group. Cholesterol sulfate and p-cresol sulfate were identified as differential metabolites between the T1 and T3 groups. It suggested that 0.4% of AGE supplementation significantly downregulated the levels of p-cresol sulfate and cholesterol sulfate (p < 0.05) and the hepatic 3-hydroxy-3-methylglutaryl-CoA reductase (HMGR) activity compared with the control. Our present study demonstrates that dietary supplementation with AGE can improve the quality and flavor by increasing the IMP and PUFA content in the muscle of Wenchang broilers. Furthermore, dietary AGE supplementation with 0.4% can regulate the cholesterol metabolism of Wenchang broilers.
本研究探讨了藤茶（显齿蛇葡萄）提取物（AGE）对文昌肉鸡的肉质、肠道微生物群和盲肠内容物代谢产物的影响。选取240只70日龄的雌性文昌肉鸡，随机分为四组，每组12只，设五个重复。肉鸡饲喂玉米 - 大豆基础日粮，并分别添加0（T1）、0.2%（T2）、0.4%（T3）和0.6%（T4）的AGE，直至124日龄。整个饲养试验持续54天。结果表明，随着日粮中AGE添加量的增加，肉鸡血清中总甘油三酯和低密度脂蛋白胆固醇含量呈线性下降（p < 0.05）。T3组和T4组大腿和胸肌的a*值高于T1组（p < 0.05）。此外，日粮中添加AGE可使大腿和胸肌的剪切力和滴水损失呈线性下降（p < 0.05）。与T1组相比，添加AGE显著提高了大腿和胸肌中肌苷酸（IMP）的含量（p < 0.05）。此外，观察到T3组大腿和胸肌中总不饱和脂肪酸（USFA）、多不饱和脂肪酸（PUFA）以及不饱和脂肪酸与饱和脂肪酸的比例（USFA:SFA）均有所增加（p < 0.05）。T3组中拟杆菌门的丰度较高（p < 0.05），厚壁菌门的丰度较低（p < 0.05）。与T1组相比，T3组中粪杆菌的丰度显著降低（p < 0.05）。硫酸胆固醇和对甲酚硫酸盐被鉴定为T1组和T3组之间的差异代谢产物。结果表明，与对照组相比，添加0.4%的AGE可显著下调硫酸对甲酚和硫酸胆固醇的水平（p < 0.05）以及肝脏3 - 羟基 - 3 - 甲基戊二酰辅酶A还原酶（HMGR）的活性。我们目前的研究表明，日粮中添加AGE可通过增加文昌肉鸡肌肉中的IMP和PUFA含量来改善肉质和风味。此外，添加0.4%的AGE可调节文昌肉鸡的胆固醇代谢。
