Zhou Shun, Yang Qiuhong, Dong Jing, Liu Yongtao, Xu Ning, Yang Yibin, Ai Xiaohui
Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan 430223, China.
Key Laboratory of Control of Quality and Safety for Aquatic Products, Ministry of Agriculture, Beijing 100071, China.
Animals (Basel). 2022 Jun 30;12(13):1685. doi: 10.3390/ani12131685.
Monogeneans are a serious threat to the development of aquaculture due to the severe economic losses they cause. The prevention and treatment of this disease are increasingly difficult because of the environmental and health concerns caused by the use of chemical anthelmintics and the emergence of drug resistance. It is thus necessary to search for effective alternatives for the treatment of monogenean infections. In the current study, anthelmintic efficacy of 16 selected essential oils (EOs) was investigated using the goldfish ()- model. The screening experiment indicated that palmarosa oil and curcuma oil had satisfactory anthelmintic activity against with EC values of 10 and 12 mg/L after 24-h exposure, respectively. The in vivo and in vitro assays indicated anthelmintic efficacy of palmarosa oil against was in a time and dose-dependent manner. Interestingly, curcuma oil showed an anesthetic effect on , and its anthelmintic activity was dose-dependent rather than time-dependent in the concentration range tested in this study. Additionally, the 24-h LC (50% lethal concentration) against goldfish of these two EOs was 8.19-fold and 5.54-fold higher than their corresponding EC (50% effective concentration) against , respectively. Moreover, exposure to these two EOs at 100% effective concentration against had no serious physiological and histopathological influence on goldfish. These results demonstrated a high safety for goldfish of these two EOs. Overall, palmarosa oil and curcuma oil could be potential candidates for the treatment of infections in aquaculture.
单殖吸虫由于会造成严重的经济损失，对水产养殖的发展构成了严重威胁。由于使用化学驱虫剂引发的环境和健康问题以及耐药性的出现，这种疾病的预防和治疗变得越来越困难。因此，有必要寻找治疗单殖吸虫感染的有效替代方法。在当前的研究中，使用金鱼（）模型研究了16种选定的精油（EOs）的驱虫效果。筛选实验表明，玫瑰草油和姜黄油对具有令人满意的驱虫活性，暴露24小时后，其EC值分别为10和12 mg/L。体内和体外试验表明，玫瑰草油对的驱虫效果呈时间和剂量依赖性。有趣的是，姜黄油对表现出麻醉作用，在本研究测试的浓度范围内，其驱虫活性呈剂量依赖性而非时间依赖性。此外，这两种精油对金鱼的24小时LC（50%致死浓度）分别比它们对的相应EC（50%有效浓度）高8.19倍和5.54倍。此外，以100%有效浓度暴露于这两种精油对金鱼没有严重的生理和组织病理学影响。这些结果表明这两种精油对金鱼具有很高的安全性。总体而言，玫瑰草油和姜黄油可能是治疗水产养殖中感染的潜在候选药物。
