人乳中低分子量肽的提取、分离与鉴定
Extraction, Isolation and Identification of Low Molecular Weight Peptides in Human Milk.
作者信息
Xiao Hailong, Jiang He, Tu Haiyun, Jia Yanbo, Wang Hongqing, Lü Xin, Fang Ruosi, Xiao Gongnian
机构信息
Key Laboratory of Agricultural Products Chemical and Biological Processing Technology, Zhejiang University of Science and Technology, Hangzhou 310023, China.
Hangzhou Institute for Food and Drug Control, Hangzhou 310017, China.
出版信息
Foods. 2022 Jun 22;11(13):1836. doi: 10.3390/foods11131836.
Human milk contains numerous free low molecular weight peptides (LMWPs), which may play an important role in infant health and growth. The bioactivities of LMWPs are determined by their structures, especially the amino acid sequences. In the present study, 81 human milk samples were collected and purified by cation-exchange solid-phase extraction (SPE). Ultra-high performance liquid chromatography coupled to quadrupole time-of-flight mass spectrometry (UPLC-QTOF-MS) was used for the separation and detection of free LMWPs in human milk. A total of 56 LMWPs were identified and quantified. These LMWPs were mainly derived from 3 regions of β-casein, which were the amino acid fragments of 16-40, 85-110, and 205-226. The predominant LMWPs were RETIESLSSSEESITEYK, RETIESLSSSEESITEYKQKVEKVK, ETIESLSSSEESITEYK, TQPLAPVHNPIS, and QPLAPVHNPISV with molecular weights of 2247.9573, 2860.2437, 2091.8591, 1372.7666, and 1271.7212, respectively. The results indicated that the technique based on SPE and UPLC-QTOF-MS might greatly facilitate the analysis of LMWPs in human milk.
人乳中含有大量游离的低分子量肽（LMWP），它们可能在婴儿健康和生长中发挥重要作用。LMWP的生物活性由其结构决定，尤其是氨基酸序列。在本研究中，收集了81份人乳样本，并通过阳离子交换固相萃取（SPE）进行纯化。采用超高效液相色谱-四极杆飞行时间质谱联用（UPLC-QTOF-MS）对人乳中的游离LMWP进行分离和检测。共鉴定并定量了56种LMWP。这些LMWP主要来源于β-酪蛋白的3个区域，即16-40、85-110和205-226的氨基酸片段。主要的LMWP分别是RETIESLSSSEESITEYK、RETIESLSSSEESITEYKQKVEKVK、ETIESLSSSEESITEYK、TQPLAPVHNPIS和QPLAPVHNPISV，分子量分别为2247.9573、2860.2437、2091.8591、1372.7666和1271.7212。结果表明，基于SPE和UPLC-QTOF-MS的技术可能极大地促进人乳中LMWP的分析。
Zotero 插件