Elrais Amina Mohamed, Arab Walid S, Sallam Khalid Ibrahim, Elmegid Walaa Abd, Elgendy Fatma, Elmonir Walid, Imre Kálmán, Morar Adriana, Herman Viorel, Elaadli Haitham
Department of Food Hygiene and Control, Faculty of Veterinary Medicine, Benha University, Benha 13511, Egypt.
Department of Food Hygiene and Control, Faculty of Veterinary Medicine, Mansura University, Mansura 35516, Egypt.
Foods. 2022 Jun 26;11(13):1890. doi: 10.3390/foods11131890.
() and () are frequently reported pathogens in humans and poultry, respectively. Nevertheless, the source of is still unclear. This study aimed to detect spp. in chicken carcasses and to assess the antibiogram and the virulence genes of isolates. Three hundred chicken meat samples (100 each of chicken breast, liver, and gizzard), besides 60 swab samples from chicken processing surfaces, were collected from retail shops in Qalyubia Governorate, Egypt, and examined for the prevalence of and . The 16S rRNA of three and two isolates were sequenced to determine the genetic relationship between these two spp. Of the 300 chicken samples tested, 16 (5.33%) and 14 (4.67%) were positive for and , respectively. Multiplex PCR revealed that the virulence genes vacuolating cytotoxin (), cytotoxin-associated gene A (), and restriction endonuclease-replacing gene A () were detected in 66.7%, 77.8%, and 100% of strains tested, respectively. showed the highest resistance for clarithromycin, while exhibited the highest resistance towards erythromycin and ciprofloxacin. The study concluded that the chicken meat and giblets are potential sources of the virulent and antimicrobial-resistant strains of of human origin.
（某菌1）和（某菌2）分别是人类和家禽中经常报道的病原体。然而，（某菌2）的来源仍不清楚。本研究旨在检测鸡胴体中的（某菌2）菌株，并评估（某菌2）分离株的抗菌谱和毒力基因。从埃及盖勒尤比省的零售店收集了300份鸡肉样本（鸡胸、肝脏和砂囊各100份），以及60份来自鸡肉加工表面的拭子样本，检测（某菌1）和（某菌2）的流行情况。对3株（某菌1）和2株（某菌2）分离株的16S rRNA进行测序，以确定这两种（某菌）菌株之间的遗传关系。在检测的300份鸡肉样本中，（某菌1）和（某菌2）的阳性样本分别为16份（5.33%）和14份（4.67%）。多重PCR显示，在检测的（某菌2）菌株中，空泡细胞毒素（vacA）、细胞毒素相关基因A（cagA）和限制性内切酶替代基因A（rdtA）毒力基因的检出率分别为66.7%、77.8%和100%。（某菌2）对克拉霉素的耐药性最高，而（某菌1）对红霉素和环丙沙星的耐药性最高。该研究得出结论，鸡肉和内脏是人类源毒力和耐抗菌药物（某菌2）菌株的潜在来源。
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