Choi Minseok, Moon SeongRyeol, Eom Hyo Jin, Lim Seung Mook, Kim Yon Hui, Nam Seungyoon
College of Medicine, Gachon University, Incheon 21565, Korea.
Department of Health Sciences and Technology, Gachon Advanced Institute for Health Sciences and Technology (GAIHST), Gachon University, Incheon 21999, Korea.
Cancers (Basel). 2022 Jun 28;14(13):3173. doi: 10.3390/cancers14133173.
Gastric cancer (GC) has the highest occurrence and fourth-highest mortality rate of all cancers in Korea. Although survival rates are improving with the development of diagnosis and treatment methods, the five-year survival rate for stage 4 GC in Korea remains <10%. Therefore, it is important to identify candidate prognostic factors for predicting poor prognosis. PRNP is a gene encoding the prion protein PrP, which has been noted for its role in the nervous system and is known to be upregulated in various cancers and associated with both cell proliferation and metastasis. However, the value of PRNP as a prognostic factor for Korean GC patients remains unclear. Here, we analyzed the relationship between PRNP expression and survival in three independent datasets for Korean patients with GC as well as the TCGA-STAD dataset. Survival analysis indicates that high levels of PRNP expression are associated with poor overall survival of patients with GC. Gene set enrichment analysis showed that PRNP is associated with epithelial mesenchymal transition and Hedgehog signaling. In addition, proliferation of GC cell lines was inhibited after siRNA-mediated knockdown of PRNP. In conclusion, our study suggests a potential role for PRNP as a candidate prognostic factor for patients with GC.
在韩国，胃癌（GC）的发病率最高，在所有癌症中的死亡率排名第四。尽管随着诊断和治疗方法的发展，生存率有所提高，但韩国IV期胃癌的五年生存率仍低于10%。因此，识别预测预后不良的候选预后因素很重要。PRNP是一种编码朊病毒蛋白PrP的基因，该蛋白因其在神经系统中的作用而受到关注，已知在各种癌症中上调，并与细胞增殖和转移相关。然而，PRNP作为韩国胃癌患者预后因素的价值仍不清楚。在此，我们分析了PRNP表达与三个韩国胃癌患者独立数据集以及TCGA-STAD数据集生存情况之间的关系。生存分析表明，PRNP高表达与胃癌患者总体生存不良相关。基因集富集分析显示，PRNP与上皮间质转化和Hedgehog信号通路相关。此外，siRNA介导的PRNP敲低后，胃癌细胞系的增殖受到抑制。总之，我们的研究表明PRNP作为胃癌患者候选预后因素具有潜在作用。
