Guangdong Provincial Key Laboratory of Eco-Circular Agriculture, Guangzhou 510642, China.
Tea Research Institute, Guangdong Academy of Agricultural Sciences/Guangdong Key Laboratory of Tea Plant Resources Innovation & Utilization, Guangzhou 510640, China.
Int J Mol Sci. 2022 Jun 23;23(13):6965. doi: 10.3390/ijms23136965.
Silicon (Si) has a multifunctional role in improving plant growth and enhancing plant disease resistance, but its mechanisms are not fully understood. In this study, we investigated the impacts of silicon application on the control of bacterial wilt and elucidated the molecular mechanisms using transcriptome sequencing. Compared to non-Si treatment, Si application (0.5-2 mM) significantly reduces tomato bacterial wilt index by 46.31-72.23%. However, Si does not influence the growth of . Si application negatively influences exopolysaccharide (EPS) synthesis and biofilm formation. Transcriptome analysis showed that Si treatment significantly downregulates the expression of virulence genes' transcriptional regulator (), EPS synthesis-related genes ( and ), and type III effectors (, , and ) in . In addition, Si remarkably upregulates the expression of twitch motor-related genes (, , , and ). These findings suggest that silicon-suppressed tomato wilt incidence may be due to the regulation of the virulence-related genes of by Si. Our research adds new knowledge to the application of Si in the field of disease control.
硅（Si）在促进植物生长和增强植物抗病性方面具有多种功能，但其机制尚不完全清楚。在本研究中，我们研究了硅的应用对防治细菌性萎蔫病的影响，并通过转录组测序阐明了其分子机制。与非硅处理相比，硅（0.5-2 mM）的应用显著降低了番茄细菌性萎蔫病指数 46.31-72.23%。然而，硅并不影响 的生长。硅的应用对胞外多糖（EPS）的合成和生物膜形成有负面影响。转录组分析表明，硅处理显著下调了 中致病相关基因转录调控因子（）、EPS 合成相关基因（和）和 III 型效应子（、、和）的表达。此外，硅还显著上调了扭缠运动相关基因（、、、和）的表达。这些发现表明，硅可能通过调控 中与致病相关的基因来抑制番茄萎蔫病的发生。我们的研究为硅在病害防治领域的应用提供了新的知识。
