College of Agriculture, Guangxi University, Nanning 530004, China.
Institute of Vegetable Research, Guangxi Academy of Agricultural Sciences, Nanning 530004, China.
Int J Mol Sci. 2022 Jun 23;23(13):6999. doi: 10.3390/ijms23136999.
Soluble solids content (SSC) is an important quality trait of wax gourd, but reports about its regulatory genes are scarce. In this study, the SSC regulatory gene in wax gourd was mined via quantitative trait locus (QTL) mapping based on high-density genetic mapping containing 12 linkage groups (LG) and bulked segregant analysis (BSA)-seq. QTL mapping and BSA-seq revealed for the first time that the SSC QTL (107.658-108.176 cM) of wax gourd was on Chr2 (LG2). The interpretable phenotypic variation rate and maximum LOD were 16.033% and 6.454, respectively. The QTL interval contained 13 genes. Real-time fluorescence quantitative expression analysis, functional annotation, and sequence analysis suggested that , named , was a candidate regulatory gene of the SSC in wax gourd. Functional annotation of this gene showed that it codes for a NADP-dependent malic enzyme. According to sequence variation, an InDel marker was developed for molecular marker-assisted breeding of wax gourd. This study will lay the foundation for future studies regarding breeding and understanding genetic mechanisms of wax gourd.
可溶性固形物含量（SSC）是冬瓜的一个重要品质性状，但关于其调控基因的报道却很少。本研究通过含有 12 个连锁群（LG）的高密度遗传图谱和分离群体分组分析法（BSA-seq）进行数量性状位点（QTL）定位，挖掘了冬瓜的 SSC 调控基因。QTL 定位和 BSA-seq 首次揭示，冬瓜 SSC 基因（107.658-108.176 cM）位于 Chr2（LG2）上。可解释的表型变异率和最大 LOD 分别为 16.033%和 6.454。该 QTL 区间包含 13 个基因。实时荧光定量表达分析、功能注释和序列分析表明，命名为 的基因是冬瓜 SSC 的候选调控基因。该基因的功能注释表明它编码一个 NADP 依赖性苹果酸酶。根据 序列变异，开发了一个 InDel 标记，用于冬瓜的分子标记辅助育种。本研究将为冬瓜的育种和遗传机制研究奠定基础。
