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比较来源于 ES 细胞和斑马鱼视网膜的类器官对 CXCR4 拮抗剂 AMD3100 的反应。

Comparison of the Response to the CXCR4 Antagonist AMD3100 during the Development of Retinal Organoids Derived from ES Cells and Zebrafish Retina.

机构信息

State Key Laboratory of Ophthalmology, Guangdong Provincial Key Laboratory of Ophthalmology and Visual Science, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou 510060, China.

出版信息

Int J Mol Sci. 2022 Jun 25;23(13):7088. doi: 10.3390/ijms23137088.

DOI:10.3390/ijms23137088
PMID:35806093
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9266567/
Abstract

Retinal organoids generated from human embryonic stem cells or iPSCs recreate the key structural and functional features of mammalian retinal tissue in vitro. However, the differences in the development of retinal organoids and normal retina in vivo are not well defined. Thus, in the present study, we analyzed the development of retinal organoids and zebrafish retina after inhibition of CXCR4, a key role in neurogenesis and optic nerve development, with the antagonist AMD3100. Our data indicated that CXCR4 was mainly expressed in ganglion cells in retinal organoids and was rarely expressed in amacrine or photoreceptor cells. AMD3100 treatment reduced the retinal organoid generation ratio, impaired differentiation, and induced morphological changes. Ganglion cells, amacrine cells, and photoreceptors were decreased and abnormal locations were observed in organoids treated with AMD3100. Neuronal axon outgrowth was also damaged in retinal organoids. Similarly, a decrease of ganglion cells, amacrine cells, and photoreceptors and the distribution of neural outgrowth was induced by AMD3100 treatment in zebrafish retina. However, abnormal photoreceptor ensembles induced by AMD3100 treatment in the organoids were not detected in zebrafish retina. Therefore, our study suggests that although retinal organoids might provide a reliable model for reproducing a retinal developmental model, there is a difference between the organoids and the retina in vivo.

摘要

从人类胚胎干细胞或 iPS 细胞中生成的视网膜类器官在体外重现了哺乳动物视网膜组织的关键结构和功能特征。然而，视网膜类器官和体内正常视网膜的发育差异尚未得到很好的定义。因此，在本研究中，我们分析了 CXCR4 拮抗剂 AMD3100 抑制神经发生和视神经发育的关键因子后，视网膜类器官和斑马鱼视网膜的发育情况。我们的数据表明，CXCR4 主要在视网膜类器官的神经节细胞中表达，在无长突细胞或光感受器细胞中很少表达。AMD3100 处理降低了视网膜类器官的生成比例，损害了分化，并诱导了形态变化。用 AMD3100 处理的类器官中神经节细胞、无长突细胞和光感受器细胞减少，并且观察到异常位置。视网膜类器官中的神经元轴突生长也受到损害。类似地，AMD3100 处理也导致斑马鱼视网膜中的神经节细胞、无长突细胞和光感受器细胞减少以及神经生长的分布受损。然而，在用 AMD3100 处理的类器官中诱导的异常光感受器集合在斑马鱼视网膜中未被检测到。因此，我们的研究表明，尽管视网膜类器官可能提供了一种可靠的模型来重现视网膜发育模型，但类器官和体内视网膜之间存在差异。

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2
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Stem Cell Reports. 2020 Jul 14;15(1):67-79. doi: 10.1016/j.stemcr.2020.05.007. Epub 2020 Jun 11.
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8
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9
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