Key Laboratory of Preservation Engineering of Agricultural Products, Ningbo Academy of Agricultural Sciences, Institute of Agricultural Products Processing, Ningbo 315040, China.
Key Laboratory of Agro-Products Processing Ministry of Agriculture, Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
Int J Mol Sci. 2022 Jul 4;23(13):7421. doi: 10.3390/ijms23137421.
The Yongyou series of indica-japonica hybrid rice has excellent production potential and storage performance. However, little is known about the underlying mechanism of its storage resistance. In this study, Yongyou 1540 rice () was stored at different temperatures, and the storability was validated though measuring nutritional components and apparent change. In addition, a broad-targeted metabolomic approach coupled with liquid chromatography-mass spectrometry was applied to analyze the metabolite changes. The study found that under high temperature storage conditions (35 °C), Yongyou 1540 was not significantly worse in terms of fatty acid value, whiteness value, and changes in electron microscope profile. A total of 19 key differential metabolites were screened, and lipid metabolites related to palmitoleic acid were found to affect the aging of rice. At the same time, two substances, guanosine 3',5'-cyclophosphate and pipecolic acid, were beneficial to enhance the resistance of rice under harsh storage conditions, thereby delaying the deterioration of its quality and maintaining its quality. Significant regulation of galactose metabolism, alanine, aspartate and glutamate metabolism, butyrate metabolism, and arginine and proline metabolism pathways were probably responsible for the good storage capacity of Yongyou 1540.
籼粳杂交稻甬优系列具有优异的生产潜力和贮藏性能。然而，其耐贮藏的机制还知之甚少。本研究以甬优 1540 水稻为材料，在不同温度下贮藏，通过测定营养成分和表观变化来验证其贮藏稳定性。此外，采用广靶代谢组学方法结合液相色谱-质谱联用技术分析了代谢物的变化。研究发现，在高温贮藏条件（35°C）下，甬优 1540 的脂肪酸值、白度值和电镜形貌变化均无明显差异。共筛选出 19 个关键差异代谢物，发现与棕榈油酸相关的脂质代谢物影响水稻的衰老。同时，两种物质环磷酸鸟苷和哌啶酸有利于增强水稻在恶劣贮藏条件下的抗性，从而延缓其品质劣变，保持其品质。推测甬优 1540 具有良好的贮藏能力可能与半乳糖代谢、丙氨酸、天冬氨酸和谷氨酸代谢、丁酸代谢以及精氨酸和脯氨酸代谢途径的显著调控有关。
