Rice Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China.
Guangdong Key Laboratory of New Technology in Rice Breeding, Guangzhou 510640, China.
Int J Mol Sci. 2022 Jul 4;23(13):7440. doi: 10.3390/ijms23137440.
Although it is known that rice 14-3-3 family genes are involved in various defense responses, the functions of in response to diseases have not been reported. Here, we showed that the transcription of was significantly induced by leaf blast infection, and the overexpression of quantitatively enhanced resistance to leaf blast and bacterial blight in rice. Further analysis showed that the expression levels of salicylic acid (SA) pathway-associated genes (, , and ) in the -overexpressing plants, were higher than those in wild-type plants after inoculation with the blast isolate ( Barr). In addition, the expression level of was significantly induced after SA treatment, and higher endogenous SA levels were observed in the -overexpressing plants compared with that in wild-type plants, especially after blast challenge. Taken together, these results suggest that positively regulates leaf blast and bacterial blight resistance in rice via the SA-dependent signaling pathway.
尽管已知水稻 14-3-3 家族基因参与各种防御反应，但关于其对疾病的反应功能尚未有报道。在这里，我们发现转录物的表达在叶片稻瘟病感染后显著上调，且过量表达在水稻中定量增强了对叶片稻瘟病和细菌性条斑病的抗性。进一步分析表明，在接种稻瘟病菌株（Barr）后，过表达植株中水杨酸（SA）途径相关基因（、、和）的表达水平高于野生型植株。此外，在 SA 处理后，的表达水平显著上调，并且在过表达植株中观察到比野生型植株更高的内源性 SA 水平，尤其是在受到稻瘟病的挑战后。综上所述，这些结果表明在水稻中通过 SA 依赖的信号通路正向调控叶片稻瘟病和细菌性条斑病抗性。
