State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, China.
The Hubei Key Lab of Plant Pathology, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, China.
Int J Mol Sci. 2022 Jul 4;23(13):7441. doi: 10.3390/ijms23137441.
Codon usage bias (CUB) could reflect co-evolutionary changes between viruses and hosts in contrast to plant and animal viruses, and the systematic analysis of codon usage among the mycoviruses that infect plant pathogenic fungi is limited. We performed an extensive analysis of codon usage patterns among 98 characterized RNA mycoviruses from eight phytopathogenic fungi. The GC and GC3s contents of mycoviruses have a wide variation from 29.35% to 64.62% and 24.32% to 97.13%, respectively. Mycoviral CUB is weak, and natural selection plays a major role in the formation of mycoviral codon usage pattern. In this study, we demonstrated that the codon usage of mycoviruses is similar to that of some host genes, especially those involved in RNA biosynthetic process and transcription, suggesting that CUB is a potential evolutionary mechanism that mycoviruses adapt to in their hosts.
密码子使用偏性（CUB）可以反映病毒和宿主之间的共同进化变化，而与植物和动物病毒相比，对感染植物病原真菌的真菌病毒的密码子使用进行系统分析的研究则较为有限。我们对来自 8 种植物病原真菌的 98 种特征 RNA 真菌病毒的密码子使用模式进行了广泛分析。真菌病毒的 GC 和 GC3s 含量变化范围较大，分别为 29.35%至 64.62%和 24.32%至 97.13%。真菌病毒的 CUB 较弱，自然选择在形成真菌病毒密码子使用模式方面起着主要作用。在这项研究中，我们证明了真菌病毒的密码子使用与某些宿主基因相似，尤其是那些涉及 RNA 生物合成过程和转录的基因，这表明 CUB 是真菌病毒适应宿主的潜在进化机制。
