Biotechnical Faculty, University of Ljubljana, Jamnikarjeva 101, 1000 Ljubljana, Slovenia.
Faculty of Chemistry and Chemical Technology, University of Ljubljana, Večna Pot, 1000 Ljubljana, Slovenia.
Int J Mol Sci. 2022 Jul 5;23(13):7460. doi: 10.3390/ijms23137460.
Tetraethyl-orthosilicate (TEOS)-based nanoparticles are most extensively used as a silica-based hemoglobin carrier system. However, TEOS-based nanoparticles induce adverse effects on the hemoglobin structure. Therefore, a heulandite-calcium-based carrier was investigated as a novel silica-based hemoglobin carrier system. The heulandite-calcium mesoporous aluminosilicate particles (MSPs) were fabricated by a patented tribo-mechanical activation process, according to the manufacturer, and its structure was assessed by X-ray diffraction analysis. Upon hemoglobin encapsulation, alternation in the secondary and tertiary structure was observed. The hemoglobin-particle interactions do not cause heme degradation or decreased activity. Once encapsulated inside the particle pores, the hemoglobin shows increased thermal stability, and higher loading capacity per gram of particles (by a factor of >1.4) when compared to TEOS-based nanoparticles. Futhermore, we introduced a PEGlyted lipid bilayer which significantly decreases the premature hemoglobin release and increases the colloidal stability. The newly developed hemoglobin carrier shows no cytotoxicity to human umbilical vein endothelial cells (HUVEC).
基于正硅酸乙酯（TEOS）的纳米颗粒最广泛地用作基于硅的血红蛋白载体系统。然而，基于 TEOS 的纳米颗粒会对血红蛋白结构产生不利影响。因此，研究了沸石钙基载体作为新型基于硅的血红蛋白载体系统。据制造商称，通过专利的摩擦机械活化工艺制备了沸石钙介孔铝硅酸盐颗粒（MSP），并通过 X 射线衍射分析评估了其结构。血红蛋白包封后，观察到二级和三级结构的改变。血红蛋白-颗粒相互作用不会导致卟啉降解或活性降低。一旦封装在颗粒孔内，血红蛋白表现出更高的热稳定性和每克颗粒更高的载量（增加了 1.4 倍以上），与基于 TEOS 的纳米颗粒相比。此外，我们引入了聚乙二醇化脂质双层，这大大降低了血红蛋白的过早释放并提高了胶体稳定性。新开发的血红蛋白载体对人脐静脉内皮细胞（HUVEC）没有细胞毒性。
