Kanasugi Kazuya, Eguchi Hiroaki, Ohgoe Yasuharu, Manome Yoshinobu, Alanazi Ali, Hirakuri Kenji
Department of Electrical and Electronic Engineering, Faculty of Engineering, Tokyo Denki University, 5 Senju Asahi-cho, Adachi-ku, Tokyo 120-8551, Japan.
Division of Electronic Engineering, Faculty of Science and Engineering, Tokyo Denki University, Ishizaka Hatoyama, Saitama 350-0394, Japan.
Materials (Basel). 2022 Jun 24;15(13):4466. doi: 10.3390/ma15134466.
In this study, correlation of cell proliferation with surface properties of the polymer-like carbon (PLC) films of different thicknesses prepared by radio-frequency plasma CVD are investigated. Four PLC samples were prepared via radio frequency plasma chemical vapor deposition on Si substrates. Each PLC film was analyzed using spectroscopic ellipsometry to determine its thickness, refractive index (n), and extinction coefficient (k); the thickness ranged from 29.0 to 356.5 nm. Based on their n−k plots, all the samples were classified as PLC-type films. The biological response of the PLC films was evaluated in vitro using a cell culture. The samples with relatively thick PLC films (>300 nm) exhibited stronger cell proliferation properties than those with thinner films. Moreover, the results of the surface analysis showed no significant differences in the surface composition of those PLC samples, as analyzed using X-ray photoelectron spectroscopy, but that as the PLC films became thicker, their surfaces became rougher on the nanoscale and their wettability improved. Overall, this study showed that careful control of the film growth of PLC films, which affects their surface properties, is essential for their use in bio-interface applications.
在本研究中，对通过射频等离子体化学气相沉积制备的不同厚度类聚合物碳（PLC）薄膜的表面性质与细胞增殖之间的相关性进行了研究。通过射频等离子体化学气相沉积在硅衬底上制备了四个PLC样品。使用光谱椭偏仪对每个PLC薄膜进行分析，以确定其厚度、折射率（n）和消光系数（k）；厚度范围为29.0至356.5纳米。根据它们的n-k图，所有样品均被归类为PLC型薄膜。使用细胞培养在体外评估PLC薄膜的生物学反应。具有相对较厚PLC薄膜（>300纳米）的样品比具有较薄薄膜的样品表现出更强的细胞增殖特性。此外，表面分析结果表明，使用X射线光电子能谱分析，那些PLC样品的表面组成没有显著差异，但随着PLC薄膜变厚，其表面在纳米尺度上变得更粗糙，润湿性得到改善。总体而言，本研究表明，仔细控制影响其表面性质的PLC薄膜的生长对于其在生物界面应用中的使用至关重要。
