Stoica Adrian, Manakhov Anton, Polčák Josef, Ondračka Pavel, Buršíková Vilma, Zajíčková Renata, Medalová Jiřina, Zajíčková Lenka
Plasma Technologies, CEITEC-Central European Institute of Technology, Masaryk University, Kotlářská, 2, Brno 61137, Czech Republic.
Fabrication and Characterisation of Nanostructures, CEITEC-Central European Institute of Technology, Brno University of Technology, Technická, 2, Brno, Czech Republic.
Biointerphases. 2015 Jun 12;10(2):029520. doi: 10.1116/1.4920978.
Recently, diamondlike carbon (DLC) thin films have gained interest for biological applications, such as hip and dental prostheses or heart valves and coronary stents, thanks to their high strength and stability. However, the biocompatibility of the DLC is still questionable due to its low wettability and possible mechanical failure (delamination). In this work, DLC:N:O and DLC: SiOx thin films were comparatively investigated with respect to cell proliferation. Thin DLC films with an addition of N, O, and Si were prepared by plasma enhanced CVD from mixtures of methane, hydrogen, and hexamethyldisiloxane. The films were optically characterized by infrared spectroscopy and ellipsometry in UV-visible spectrum. The thickness and the optical properties were obtained from the ellipsometric measurements. Atomic composition of the films was determined by Rutherford backscattering spectroscopy combined with elastic recoil detection analysis and by x-ray photoelectron spectroscopy. The mechanical properties of the films were studied by depth sensing indentation technique. The number of cells that proliferate on the surface of the prepared DLC films and on control culture dishes were compared and correlated with the properties of as-deposited and aged films. The authors found that the level of cell proliferation on the coated dishes was high, comparable to the untreated (control) samples. The prepared DLC films were stable and no decrease of the biocompatibility was observed for the samples aged at ambient conditions.
最近，类金刚石碳（DLC）薄膜因其高强度和稳定性而在生物应用领域受到关注，如髋关节和牙科假体、心脏瓣膜及冠状动脉支架等。然而，由于其低润湿性和可能的机械失效（分层），DLC的生物相容性仍存在疑问。在这项工作中，对DLC:N:O和DLC:SiOx薄膜的细胞增殖情况进行了比较研究。通过等离子体增强化学气相沉积法，以甲烷、氢气和六甲基二硅氧烷的混合物为原料制备了添加氮、氧和硅的DLC薄膜。通过红外光谱和紫外-可见光谱中的椭偏仪对薄膜进行光学表征。从椭偏测量中获得薄膜的厚度和光学性质。通过卢瑟福背散射光谱结合弹性反冲检测分析以及X射线光电子能谱确定薄膜的原子组成。采用深度传感压痕技术研究薄膜的力学性能。比较了在制备的DLC薄膜表面和对照培养皿上增殖的细胞数量，并将其与沉积态和老化薄膜的性能相关联。作者发现，涂覆培养皿上的细胞增殖水平较高，与未处理（对照）样品相当。制备的DLC薄膜稳定，在环境条件下老化的样品未观察到生物相容性下降。
