School of Pharmaceutical Sciences, Hunan University of Medicine, Huaihua 418000, China.
National Institutes for Food and Drug Control, Beijing 100050, China.
Molecules. 2022 Jun 23;27(13):4038. doi: 10.3390/molecules27134038.
(Roxb.) Ridsd. et Badh. F. (CT) belongs to the Rubiaceae family. Its dried leaves are widely used in traditional Chinese medicine to treat enteritis, dysentery, toothache, furuncles, swelling, traumatic injury, fracture, bleeding, and scalding. In order to further clarify the unknown chemical composition of CT, a rapid strategy based on UHPLC-Q-exactive orbitrap was established for this analysis using a Thermo Scientific Hypersil GOLD aQ (100 mm × 2.1 mm, 1.9 µm) chromatographic column. The mobile phase was 0.1% formic acid water-acetonitrile, with a flow rate of 0.3 mL/min and injection volume of 2 µL; for mass spectrometry, an ESI ion source in positive and negative ion monitoring modes was adopted. A total of 135 chemicals comprising 67 chlorogenic acid derivatives, 48 flavonoids, and 20 anthocyanin derivatives were identified by comparing the mass spectrum information with standard substances, public databases, and the literature, which were all discovered for the first time in this plant. This result broadly expands the chemical composition of CT, which will contribute to understanding of its effectiveness and enable quality control.
(Roxb.) Ridsd. et Badh. F. (CT) 属于茜草科。其干叶在传统中药中被广泛用于治疗肠炎、痢疾、牙痛、痈肿、创伤、骨折、出血和烫伤。为了进一步阐明 CT 的未知化学成分，本研究采用基于 UHPLC-Q-exactive orbitrap 的快速策略，使用 Thermo Scientific Hypersil GOLD aQ（100mm×2.1mm，1.9µm）色谱柱进行分析。流动相为 0.1%甲酸水-乙腈，流速为 0.3mL/min，进样量为 2µL；质谱采用 ESI 离子源在正负离子监测模式下进行检测。通过将质谱信息与标准物质、公共数据库和文献进行比较，共鉴定出 135 种化学成分，包括 67 种绿原酸衍生物、48 种黄酮类化合物和 20 种花色苷衍生物，这些均为该植物中的首次发现。该结果广泛扩展了 CT 的化学成分，有助于了解其功效并实现质量控制。
