• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

基于网络药理学的研究探讨天然血管保护组合 DGS 治疗冠心病的潜在成分和作用机制。

Investigation Driven by Network Pharmacology on Potential Components and Mechanism of DGS, a Natural Vasoprotective Combination, for the Phytotherapy of Coronary Artery Disease.

机构信息

School of Pharmacy and Pharmaceutical Science, Shandong First Medical University and Shandong Academy of Medical Sciences, Jinan 250000, China.

School of Pharmaceutical Science, Shanxi Medical University, Taiyuan 030000, China.

出版信息

Molecules. 2022 Jun 24;27(13):4075. doi: 10.3390/molecules27134075.

DOI:10.3390/molecules27134075
PMID:35807320
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9268537/
Abstract

Phytotherapy offers obvious advantages in the intervention of Coronary Artery Disease (CAD), but it is difficult to clarify the working mechanisms of the medicinal materials it uses. DGS is a natural vasoprotective combination that was screened out in our previous research, yet its potential components and mechanisms are unknown. Therefore, in this study, HPLC-MS and network pharmacology were employed to identify the active components and key signaling pathways of DGS. Transgenic zebrafish and HUVECs cell assays were used to evaluate the effectiveness of DGS. A total of 37 potentially active compounds were identified that interacted with 112 potential targets of CAD. Furthermore, PI3K-Akt, MAPK, relaxin, VEGF, and other signal pathways were determined to be the most promising DGS-mediated pathways. NO kit, ELISA, and Western blot results showed that DGS significantly promoted NO and VEGFA secretion via the upregulation of VEGFR2 expression and the phosphorylation of Akt, Erk1/2, and eNOS to cause angiogenesis and vasodilation. The result of dynamics molecular docking indicated that Salvianolic acid C may be a key active component of DGS in the treatment of CAD. In conclusion, this study has shed light on the network molecular mechanism of DGS for the intervention of CAD using a network pharmacology-driven strategy for the first time to aid in the intervention of CAD.

摘要

植物疗法在干预冠状动脉疾病（CAD）方面具有明显优势，但难以阐明其所用药材的作用机制。DGS 是我们之前的研究中筛选出的一种天然血管保护组合，但它的潜在成分和机制尚不清楚。因此，在这项研究中，我们采用 HPLC-MS 和网络药理学方法来鉴定 DGS 的活性成分和关键信号通路。利用转基因斑马鱼和 HUVECs 细胞实验来评估 DGS 的功效。共鉴定出 37 种潜在的活性化合物，这些化合物与 CAD 的 112 个潜在靶点相互作用。此外，确定 PI3K-Akt、MAPK、松弛素、VEGF 等信号通路是 DGS 介导的最有前途的途径。NO 试剂盒、ELISA 和 Western blot 结果表明，DGS 通过上调 VEGFR2 表达和磷酸化 Akt、Erk1/2 和 eNOS，显著促进了 NO 和 VEGFA 的分泌，从而引起血管生成和血管舒张。动态分子对接结果表明，丹酚酸 C 可能是 DGS 治疗 CAD 的关键活性成分。总之，本研究首次采用网络药理学驱动的策略，阐明了 DGS 干预 CAD 的网络分子机制，为 CAD 的干预提供了一定的参考依据。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/59a6/9268537/88a1b4f1a2d8/molecules-27-04075-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/59a6/9268537/cf4caceff3ff/molecules-27-04075-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/59a6/9268537/86e9e1bca0fb/molecules-27-04075-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/59a6/9268537/687b4edb19b7/molecules-27-04075-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/59a6/9268537/43345ba596ec/molecules-27-04075-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/59a6/9268537/5c58bc20d64c/molecules-27-04075-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/59a6/9268537/3b28a2b4638f/molecules-27-04075-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/59a6/9268537/f80e925f57fc/molecules-27-04075-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/59a6/9268537/88a1b4f1a2d8/molecules-27-04075-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/59a6/9268537/cf4caceff3ff/molecules-27-04075-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/59a6/9268537/86e9e1bca0fb/molecules-27-04075-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/59a6/9268537/687b4edb19b7/molecules-27-04075-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/59a6/9268537/43345ba596ec/molecules-27-04075-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/59a6/9268537/5c58bc20d64c/molecules-27-04075-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/59a6/9268537/3b28a2b4638f/molecules-27-04075-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/59a6/9268537/f80e925f57fc/molecules-27-04075-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/59a6/9268537/88a1b4f1a2d8/molecules-27-04075-g008.jpg

相似文献

1
Investigation Driven by Network Pharmacology on Potential Components and Mechanism of DGS, a Natural Vasoprotective Combination, for the Phytotherapy of Coronary Artery Disease.基于网络药理学的研究探讨天然血管保护组合 DGS 治疗冠心病的潜在成分和作用机制。
Molecules. 2022 Jun 24;27(13):4075. doi: 10.3390/molecules27134075.
2
Uncovering the active components, prospective targets, and molecular mechanism of Baihe Zhimu decoction for treating depression using network pharmacology-based analysis.基于网络药理学分析揭示百合知母汤治疗抑郁症的活性成分、潜在靶点和分子机制。
J Ethnopharmacol. 2021 Dec 5;281:114586. doi: 10.1016/j.jep.2021.114586. Epub 2021 Aug 28.
3
Network Pharmacology and Molecular Docking Study of the Chinese Miao Medicine Sidaxue in the Treatment of Rheumatoid Arthritis.基于网络药理学和分子对接技术的苗药四方雪治疗类风湿关节炎的作用机制研究。
Drug Des Devel Ther. 2022 Feb 19;16:435-466. doi: 10.2147/DDDT.S330947. eCollection 2022.
4
Erianin, the main active ingredient of Dendrobium chrysotoxum Lindl, inhibits precancerous lesions of gastric cancer (PLGC) through suppression of the HRAS-PI3K-AKT signaling pathway as revealed by network pharmacology and in vitro experimental verification.铁皮石斛中的主要活性成分鼓槌石斛碱通过网络药理学和体外实验验证，通过抑制 HRAS-PI3K-AKT 信号通路抑制胃癌前病变（PLGC）。
J Ethnopharmacol. 2021 Oct 28;279:114399. doi: 10.1016/j.jep.2021.114399. Epub 2021 Jul 8.
5
Xiao-Luo-Wan treats propylthiouracil-induced goiter with hypothyroidism in rats through the PI3K-AKT/RAS pathways based on UPLC/MS and network pharmacology.消瘿丸通过 UPLC/MS 和网络药理学治疗丙硫氧嘧啶诱导的甲状腺功能减退大鼠甲状腺肿基于 PI3K-AKT/RAS 通路。
J Ethnopharmacol. 2022 May 10;289:115045. doi: 10.1016/j.jep.2022.115045. Epub 2022 Jan 29.
6
Deciphering the pharmacological mechanisms of Scutellaria baicalensis Georgi on oral leukoplakia by combining network pharmacology, molecular docking and experimental evaluations.基于网络药理学、分子对接和实验评估相结合的方法解析黄芩治疗口腔白斑的药理学机制。
Phytomedicine. 2022 Aug;103:154195. doi: 10.1016/j.phymed.2022.154195. Epub 2022 May 22.
7
Network Pharmacology Prediction and Molecular Docking-Based Strategy to Discover the Potential Pharmacological Mechanism of Huai Hua San Against Ulcerative Colitis.基于网络药理学预测和分子对接的方法发现槐黄散治疗溃疡性结肠炎的潜在作用机制。
Drug Des Devel Ther. 2021 Jul 28;15:3255-3276. doi: 10.2147/DDDT.S319786. eCollection 2021.
8
Identification of the active compounds and functional mechanisms of Jinshui Huanxian formula in pulmonary fibrosis by integrating serum pharmacochemistry with network pharmacology.基于血清药物化学与网络药理学整合分析金水还肝方治疗肺纤维化的活性成分及作用机制。
Phytomedicine. 2022 Jul 20;102:154177. doi: 10.1016/j.phymed.2022.154177. Epub 2022 May 21.
9
[Mechanism of astragaloside Ⅳ alleviating PC12 cell injury by activating PI3K/AKT signaling pathway: based on network pharmacology and in vitro experiments].[黄芪甲苷Ⅳ通过激活PI3K/AKT信号通路减轻PC12细胞损伤的机制：基于网络药理学和体外实验]
Zhongguo Zhong Yao Za Zhi. 2021 Dec;46(24):6465-6473. doi: 10.19540/j.cnki.cjcmm.20210902.702.
10
The potential effects and mechanisms of hispidulin in the treatment of diabetic retinopathy based on network pharmacology.基于网络药理学探讨芹黄素治疗糖尿病视网膜病变的潜在作用及机制。
BMC Complement Med Ther. 2022 May 19;22(1):141. doi: 10.1186/s12906-022-03593-2.

引用本文的文献

1
Spectroscopic, Thermodynamic and Molecular Docking Studies on Molecular Mechanisms of Drug Binding to Proteins.光谱学、热力学和分子对接研究药物与蛋白质结合的分子机制。
Molecules. 2022 Dec 1;27(23):8405. doi: 10.3390/molecules27238405.

本文引用的文献

1
Vascular dysfunction and increased cardiovascular risk in hypospadias.尿道下裂患者的血管功能障碍和心血管风险增加。
Eur Heart J. 2022 May 14;43(19):1832-1845. doi: 10.1093/eurheartj/ehac112.
2
Screening and identification of natural α-glucosidase and α-amylase inhibitors from partridge tea (Mallotus furetianus Muell-Arg) and in silico analysis.鹧鸪茶（Mallotus furetianus Muell-Arg）中天然α-葡萄糖苷酶和α-淀粉酶抑制剂的筛选与鉴定及计算机模拟分析
Food Chem. 2022 Sep 15;388:133004. doi: 10.1016/j.foodchem.2022.133004. Epub 2022 Apr 20.
3
DAVID: a web server for functional enrichment analysis and functional annotation of gene lists (2021 update).
DAVID：一个用于基因列表功能富集分析和功能注释的网络服务器（2021 更新）。
Nucleic Acids Res. 2022 Jul 5;50(W1):W216-W221. doi: 10.1093/nar/gkac194.
4
Coronary Artery Disease in Very Young Patients: Analysis of Risk Factors and Long-Term Follow-Up.极年轻患者的冠状动脉疾病：危险因素分析及长期随访
J Cardiovasc Dev Dis. 2022 Mar 11;9(3):82. doi: 10.3390/jcdd9030082.
5
The Role of Flavonoids as a Cardioprotective Strategy against Doxorubicin-Induced Cardiotoxicity: A Review.黄酮类化合物作为一种对抗多柔比星诱导的心脏毒性的心脏保护策略的作用：综述。
Molecules. 2022 Feb 15;27(4):1320. doi: 10.3390/molecules27041320.
6
Current and Future Applications of Artificial Intelligence in Coronary Artery Disease.人工智能在冠状动脉疾病中的当前及未来应用
Healthcare (Basel). 2022 Jan 26;10(2):232. doi: 10.3390/healthcare10020232.
7
Role of endothelial colony forming cells (ECFCs) Tetrahydrobiopterin (BH4) in determining ECFCs functionality in coronary artery disease (CAD) patients.内皮祖细胞（ECFCs）四氢生物蝶呤（BH4）在决定冠心病（CAD）患者中 ECFCs 功能中的作用。
Sci Rep. 2022 Feb 23;12(1):3076. doi: 10.1038/s41598-022-06758-8.
8
Challenges in Healing Wound: Role of Complementary and Alternative Medicine.伤口愈合的挑战：补充与替代医学的作用
Front Nutr. 2022 Jan 20;8:791899. doi: 10.3389/fnut.2021.791899. eCollection 2021.
9
The essential role for endothelial cell sprouting in coronary collateral growth.内皮细胞出芽在冠状动脉侧支生长中的重要作用。
J Mol Cell Cardiol. 2022 Apr;165:158-171. doi: 10.1016/j.yjmcc.2022.01.005. Epub 2022 Jan 22.
10
Vasodilatory Effect of Guanxinning Tablet on Rabbit Thoracic Aorta is Modulated by Both Endothelium-Dependent and -Independent Mechanism.冠心宁片对兔胸主动脉的舒张作用受内皮依赖性和非内皮依赖性机制的调控。
Front Pharmacol. 2021 Dec 1;12:754527. doi: 10.3389/fphar.2021.754527. eCollection 2021.