Collaborative Innovation Center of Yangtze River Delta Region Green Pharmaceuticals, Zhejiang University of Technology, Hangzhou 310014, China.
Molecules. 2022 Jun 27;27(13):4128. doi: 10.3390/molecules27134128.
Smoke-induced acute lung injury (ALI) is a grievous disease with high mortality. Despite advances in medical intervention, no drug has yet been approved by the Food and Drug Administration (FDA) for ALI. In this study, we reported that pretreatment with high-molecular-weight hyaluronan (1600 kDa, HA1600) alleviated pulmonary inflammation and injury in mice exposed to smoke and also upregulated long non-coding RNA (lncRNA) metastasis-associated lung adenocarcinoma transcript 1 (), as well as suppressor of cytokine signaling-1 (SOCS-1), in the lung tissues. Next, we overexpressed in the lungs by intratracheal administration of adenovirus cloned with cDNA and found that the survival of mice after smoke exposure was improved. Moreover, pulmonary overexpression of ameliorated smoke-induced ALI in mice and elevated the level of SOCS-1 in the lungs. In conclusion, the results pointed out that HA1600 exerted a protective effect against smoke-induced ALI through increasing the level and the subsequent SOCS-1 expression. Our study provides a potential therapeutic approach to smoke-induced ALI and a novel insight into the mechanism of action of HA1600.
烟雾诱导的急性肺损伤（ALI）是一种死亡率很高的严重疾病。尽管医学干预取得了进展，但目前还没有一种药物被食品和药物管理局（FDA）批准用于 ALI。在这项研究中，我们报告称，高分子量透明质酸（1600 kDa，HA1600）预处理可减轻暴露于烟雾中的小鼠的肺部炎症和损伤，同时上调长链非编码 RNA（lncRNA）转移相关肺腺癌转录本 1（）以及细胞因子信号转导抑制因子-1（SOCS-1）在肺组织中的表达。接下来，我们通过气管内给予携带 cDNA 的腺病毒转染来在肺部过表达 ，发现暴露于烟雾后小鼠的存活率提高。此外，肺部过表达 可改善小鼠烟雾诱导的 ALI ，并提高肺部 SOCS-1 的水平。总之，结果表明 HA1600 通过增加 水平和随后的 SOCS-1 表达对烟雾诱导的 ALI 发挥保护作用。我们的研究为烟雾诱导的 ALI 提供了一种潜在的治疗方法，并为 HA1600 的作用机制提供了新的见解。
