基于无标记适体的简单结构切换适体荧光传感器用于黄曲霉毒素 B1 的检测。
A Simple Structure-Switch Aptasensor Using Label-Free Aptamer for Fluorescence Detection of Aflatoxin B1.
机构信息
State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China.
University of Chinese Academy of Sciences, Beijing 100049, China.
出版信息
Molecules. 2022 Jul 1;27(13):4257. doi: 10.3390/molecules27134257.
Aflatoxin B1 (AFB1) is one of the mycotoxins produced by and , and it causes contamination in foods and great risk to human health. Simple sensitive detection of AFB1 is important and demanded for food safety and quality control. Aptamers can specifically bind to targets with high affinity, showing advantages in affinity assays and biosensors. We reported an aptamer structure-switch for fluorescent detection of aflatoxin B1 (AFB1), using a label-free aptamer, a fluorescein (FAM)-labeled complementary strand (FDNA), and a quencher (BHQ1)-labeled complementary strand (QDNA). When AFB1 is absent, these three strands assemble into a duplex DNA structure through DNA hybridization, making FAM close to BHQ1, and fluorescence quenching occurs. In the presence of AFB1, the aptamer binds with AFB1, instead of hybridizing with QDNA. Thus, FAM is apart from BHQ1, and fluorescence increases with the addition of AFB1. This assay allowed detection of AFB1 with a detection limit of 61 pM AFB1 and a dynamic concentration range of 61 pM to 4 μM. This aptamer-based method enabled detection of AFB1 in complex sample matrix (e.g., beer and corn flour samples).
黄曲霉毒素 B1（AFB1）是 和 产生的霉菌毒素之一，它会导致食品污染，对人类健康造成极大风险。简单灵敏地检测 AFB1 对于食品安全和质量控制非常重要和必要。适体可以与靶标特异性结合，具有亲和力测定和生物传感器方面的优势。我们报道了一种适体结构开关，用于荧光检测黄曲霉毒素 B1（AFB1），使用无标记适体、荧光素（FAM）标记的互补链（FDNA）和淬灭剂（BHQ1）标记的互补链（QDNA）。当不存在 AFB1 时，这三条链通过 DNA 杂交组装成双链 DNA 结构，使 FAM 靠近 BHQ1，发生荧光猝灭。当存在 AFB1 时，适体与 AFB1 结合，而不是与 QDNA 杂交。因此，FAM 远离 BHQ1，并且随着 AFB1 的加入，荧光增强。该测定法可以检测到 61 pM AFB1 的 AFB1，动态浓度范围为 61 pM 至 4 μM。这种基于适体的方法能够检测复杂样品基质（例如啤酒和玉米粉样品）中的 AFB1。
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