基于适配体的简单荧光分析法检测婴儿米粉中的黄曲霉毒素 B1。
A simple aptamer-based fluorescent assay for the detection of Aflatoxin B1 in infant rice cereal.
机构信息
Ministry of Agriculture Laboratory of Quality & Safety Risk Assessment for Dairy Products (Beijing), Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, PR China; College of Animal Science, Xinjiang Agricultural University, Urumchi 830052, PR China; Ministry of Agriculture - Milk and Dairy Product Inspection Center (Beijing), Beijing 100193, PR China.
Ministry of Agriculture Laboratory of Quality & Safety Risk Assessment for Dairy Products (Beijing), Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, PR China; Ministry of Agriculture - Milk and Dairy Product Inspection Center (Beijing), Beijing 100193, PR China.
出版信息
Food Chem. 2017 Jan 15;215:377-82. doi: 10.1016/j.foodchem.2016.07.148. Epub 2016 Aug 2.
A fluorescent assay for the rapid, sensitive and specific detection of Aflatoxin B1 (AFB1) was developed in this study. Initially, a DNA/DNA duplex was formed between a fluorescein-labeled AFB1 aptamer and its partially complementary DNA strand containing a quencher moiety, resulting in fluorescence quenching due to the close proximity of fluorophore and quencher. Upon the addition of AFB1, an aptamer/AFB1 complex was generated to release the quencher-modified DNA strand, thus recovered the fluorescence of fluorescein and enabled quantitative detection for AFB1 by monitoring fluorescence enhancement. Under optimized conditions, this assay exhibited a linear response to AFB1 in the range of 5-100ng/mL with a detection limit down to 1.6ng/mL. Trials of this assay in infant rice cereal with satisfactory recovery in the range of 93.0%-106.8%, demonstrate that the new assay could be a potential sensing platform for AFB1 determination in food.
本研究开发了一种用于快速、灵敏和特异性检测黄曲霉毒素 B1（AFB1）的荧光检测法。该方法首先通过荧光素标记的 AFB1 适体与其部分互补的 DNA 链形成 DNA/DNA 双链，由于荧光团和猝灭剂的接近，导致荧光猝灭。当加入 AFB1 时，形成适体/AFB1 复合物，释放出带有猝灭剂修饰的 DNA 链，从而恢复了荧光素的荧光，并通过监测荧光增强实现了对 AFB1 的定量检测。在优化条件下，该方法在 5-100ng/mL 范围内对 AFB1 呈线性响应，检测限低至 1.6ng/mL。在婴儿米粉中的试验表明，该方法的回收率在 93.0%-106.8%范围内，表明该新方法可能成为食品中 AFB1 测定的潜在传感平台。
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